石棉纤维诱发8－羟脱氧鸟苷导致DNA损伤的体外研究  

A Study of DNA Damage Induced by Asbestos Fibers in Vitro

作　　者：张建新[1] 孙成均[1] 姚群梅 梁娴[1] 柯佛心[1] 

机构地区：[1]华西医科大学公共卫生学院,云南省楚雄州医院,成都市卫生防疫站

出　　处：《华西医科大学学报》1994年第3期333-336,共4页Journal of West China University of Medical Sciences

基　　金：校青年科学基金

摘　　要：通过体外实验观察到Ｒｈｏｄｅｓｉａ温石棉，新康温石棉和ＵＩＣＣ温石棉、青石棉、铁石棉具有诱发８－羟脱氧鸟苷（８－ＯＨ－ｄＧ）产生而损伤小牛胸腺ＤＮＡ的作用，加入Ｈ＿２Ｏ＿２和（或）ＦｅＳＯ＿４可明显增强石棉纤维致８－ＯＨ－ｄＧ产生的作用。以ＵＩＣＣ青石棉纤维的作用最强，ＵＩＣＣ铁石棉纤维次之，Ｒｈｏｄｅｓｉａ温石棉、新康温石棉和ＵＩＣＣ温石棉纤维最弱。由此可见石棉纤维致８－ＯＨ－ｄＧ产生的作用与石棉种类和羟自由基的参与有关。作者认为８－ＯＨ－ｄＧ的产生可影响ＤＮＡ中腺嘌呤与胸腺嘧啶、鸟嘌呤与胞嘧啶的碱基配对，诱发ＤＮＡ点突变，与肿瘤的发生有关。The results of our in vitro studyshowed that Rhodesia chrysotile,X.K.chrysotile,UICC chrvsotne, crocidolit and amosite could causedamage to calf thymus DNA by inducing the produc-tion of 8-hydroxy-2￣'-deoxyguanosine(8-OH-dG). Af-ter adding H_2O_2 and/or FeSO_4, the production of 8-OH-dG, induced by asbestos fibers was signi ficantlyincreased. The fiher of UICC crocidolite has the mostuntoward efect,that of UICC amosite comes next,and the three chrysotile fibers are the weakest ones.The effect of asbestos fibers on the production of 8-OH-dG has some relationship with hydroxy free radi-cal and the type of asbestos, It is suggested that as-bestos fibers could induce DNA point mutationthrough A·Tand G·Ctransversion by the prodac-tion of 8-OH-dG, which is related with the carcinogen-esis of asbestos.

关 键 词：羟脱氧鸟苷 石棉 DNA损伤 纤维 

分 类 号：R730.231.1[医药卫生—肿瘤]