热动力学对比进度法 Ⅵ.单底物酶促反应的热动力学研究  

Reduced Extent Method for Thermokinetics Ⅵ. Thermokinetic Research of the Enzyme-catalyzed One-substrate Reaction

作　　者：刘劲松[1] 曾宪诚[1] 邓郁[1] 田安民[1] 

机构地区：[1]四川大学化学系,成都610064

出　　处：《化学学报》1994年第8期767-773,共7页Acta Chimica Sinica

基　　金：国家自然科学基金资助的项目

摘　　要：本文导出了单底物酶促反应的热动力学变换方程组,建立了单底物酶促反应动力学的对比进度方程和热动力学的数学模型.根据此模型,可由一次反应的热谱曲线同时解析出动力学参数(K_m,V_m)和反应的摩尔焓(△H).用热导式热量计研究了α-糜蛋白酶催化N-乙酰基-L-酪氨酸乙酯水解的热动力学.并对本文建立的热动力学研究法的特点作了详尽的讨论.Transformation equations for thermokinetics of the enzyme-catalyzed one-substrate reactions is deduced, and then, reduced extent equation for its reaction kinetics and mathematical model for its thermokinetics have been suggested, which can be used to calculate both kinetic parameters (Km, Vm) and molar enthalpy (ΔH) simultaneously by analysising a thermogram. Thermokinetics of hydrolysis of ATEE catalyzed by α-chymotrypsin was studied with a conduction calorimeter, and the characteristics of this method in this paper have been discussed in detail.

关 键 词：酶促反应 热动力学 对比进度法 

分 类 号：O643.19[理学—物理化学]