二十种混合氨基酸的质谱鉴定直接磷酰化物的正负离子FAB—MS分析  

The In situ Positive and Negative FAB-MS of the 20 Amino Acids Phosphorylated by the Dialkylphorphits

作　　者：尹应武[1] 麻远[1] 赵玉芬[1] 辛斌[2] 王光辉[2] 

机构地区：[1]清华大学化学系,北京100084 [2]中国科学院化学研究所,北京100080

出　　处：《化学学报》1994年第11期1112-1117,共6页Acta Chimica Sinica

基　　金：清华大学校内基金;国家教委青年基金;国家自然科学基金;北京中关村大型仪器联合测试基金资助的课题.

摘　　要：将二十种天然氨基酸按等摩尔量混合后,用二异丙基亚磷酸酯直接磷酰化,可以高选择性(96%)地获得N-磷酰氨基酸衍生物的混合物,负离子FAB-MS可以原位检出混合物中全部N-磷酰氨基酸,且准分子离子峰较高,可发展为混合氨基酸衍生物全分析的有效手段.正离子FAB-MS则对碱性磷酰化氨基酸的检出更有利.The Positive and Negative FAB-MS of the mixed twenty phosphorylated amino acids were reported. It was found that the triethylamine and glycerol matrix background was suppressed. By this insitu phosphorylation method, all twenty amino acids could be detected no matter how much phosphoryltion reagent was used. The basic amino acids showed much greater response to the positive FAB-MS. While the neutral and acidic amino acids were give much more intense molecular ion peaks in the negative FAB-MS. This method could apply to the qualitative detection of the amino acids in samples.

关 键 词：氨基酸 质谱 磷酰化物 FAB-MS 

分 类 号：O657.6[理学—分析化学]