缺氧对肺动脉平滑肌细胞增殖的影响及其传递途径  

Effect of Hypoxia on Proliferation of Pulmonary Arterial Smooth Muscle Cell and its Signal Transduction Pathway

作　　者：宋为[1] 蔡英年[1] 邓希贤[1] 李世强 

机构地区：[1]中国医学科学院基础医学研究所生理研究室

出　　处：《基础医学与临床》1994年第5期35-39,共5页Basic and Clinical Medicine

摘　　要：本文观察了低氧对离体培养成年牛肺动脉平滑肌细胞（ＰＡＳＭＣ）３Ｈ－ＴｄＲ参入的影响，并用异搏定、Ｈ７、ＰＭＡ和ＥＣＦ进行干预实验，对ＳＭＣ增殖中信息传递途径作了初探。结果显示，（１）缺氧２４ｈ，ＰＡＳＭＣ３Ｈ－ＴｄＲ参入显著增高。（２）异搏定及Ｈ７显著降低低氧Ｈ－ＳＭＣ３Ｈ－ＴｄＲ参入，而ＰＭＡ及ＥＣＦ显著增加ＨＳＭＣ３ＨＴｄＲ参入。（３）Ｈ－ＳＭＣ对ＰＭＡ和ＥＣＦ反应强于常氧Ｎ－ＳＭＣ；但对异搏定及Ｈ７反应弱于Ｎ－ＳＭＣ。结果提示：低氧可直接刺激成年牛ＰＡＳＭＣ增殖，其途径可能包含Ｃａ２＋内流增加和ＰＫＣ进一步活化。外源ＰＭＡ可能会进一步激活ＰＫＣ而增强ＳＭＣ增殖。ＥＧＦ促增殖作用与ＰＫＣ有一定关联。ＮＳＭＣ及Ｈ－ＳＭＣ对干预物质反应程度的差别，反映出低氧是多环节作用于细胞代谢，从而提高细胞整体代谢水平。he effect of hypoxia on incorporation of 3H-TdR into PASMC of bovine invitro was observed.Verapamil,H7,PMA ,and EGF were used for interfering ex-periments to study signal transduction pathway in PASMC prolifetation.Theresults showed that(1)after 24h of hypoxic exposure,the incorporation apparentlyincreased;(2) in hypoxic PASMC , verapaxmil and H7 could notably decrease theincorporation and PMA and EGF could notably increase the incorporation;(3)the reactions of hypoxic PASMC to PMA and EGF was more intense than that ofnormoxic PASMC, but its reactions to verapamil and H7 was weaker than that ofnormoxic PASMC.The results indicated that PASMC could proliferate under hypoxic stmulation by means of increasing Ca2+ influx and further activating PKC;foreign PMA might enhance the hypoxic proliferation by activating PKC;the hypo-xic proliferation promoted by EGF might have some relation with PKC pathway; inconsideration of the different reactive levels to signal transduction interferingbetween normoxic and hypoxic PASMCs, it is possible that hypoxia might increasethe whole metabolism of PASMC through acting many metabolic links.

关 键 词：肺动脉 平滑肌细胞 信息传递途径 缺氧环境 

分 类 号：R364.4[医药卫生—病理学]