不同色光、温度、pH对草珊瑚愈伤组织的培养效应  

STUDY ON DIFFERENT COLOUR LIGHTS TEMPERATURES AND PH VALUES ON CALLUS GROWTH AND SECONDARY METABOLITE ACCUMULATION OF SARCANDRA GLABRA

作　　者：涂艺声[1] 王碧琴[1] 江洪如[1] 奚昕[1] 丁建南[1] 

机构地区：[1]江西省科学院生物技术研究中心

出　　处：《江西科学》1994年第2期85-89,共5页Jiangxi Science

基　　金：江西省自然科学基金

摘　　要：报道了草瑚珊愈伤组织生长和次生代谢物与光、温度、ｐＨ不同的物理因子培养条件的关系。结果表明，暗培养的愈伤组织生长和代谢物含量较高，而蓝光在草珊瑚愈伤组织培养中，表现了蓝光效应，诱导出一个优良的愈伤组织系，其相对增长率和总黄酮月产率分别为７５９％，１．８６８ｇ／Ｌ。最佳培养温度为２６℃恒温，最适愈伤组织生长的培养基ｐＨ值为５．５；而最适愈伤组织代谢次级产物的生产培养基ｐＨ值为６．０。This paper reported the relationship between callus growth and secondary metabolite of Sarcandra glabra with different culture conditions of lights, temperatures and pH values. The results demonstrated that the callus growth of dark culture showed better than white light and red light; but it was the blue light effect in callus culture of Sarcandra glabra.A good callus strain was induced by blue light culture, the relative increasing rate reached seven hundred fifty nine percent and yield of flavonoid glycosides per month 1 868 mg/1, the optimum temperature was around 26℃ and the optimume pH of callus growth was 5.5 but the optimum pH of callus production was 6.0.

关 键 词：草珊瑚 愈伤组织 组织培养 光 温度 

分 类 号：Q959.133[生物学—动物学]