有色金属混合尘诱癌病变图象分析及DNA含量测定的研究  

Quantitative Analysis of Cellular Morphometry and DNA Content of Experimental Lung Cancer Lesions Induced by Non-ferrous Metal Dust

作　　者：黄润[1] 陆建波[1] 章宗籍[1] 王国英[1] 张华献[1] 

机构地区：[1]昆明医学院病理学教研室

出　　处：《昆明医学院学报》1994年第1期39-43,共5页Journal of Kunming Medical College

基　　金：云南省科委应用基础研究基金

摘　　要：采用图象分析仪对有色金属混合尘诱发肺腺癌过程中正常、增生支气管上皮、癌前病变和肺腺癌（１～４组）进行细胞形态计量及ＤＮＡ含量测定，结果显示：经逐步判别分析，从１６项几何参数中优选出对各组诊断、鉴别诊断有较大价值的７项参数，并建立判别函数方程，经回代判别分析，准确率达９６．３％；ＤＮＡ含量测定，１，２组未出现ＤＮＡ倍性＞５Ｃ细胞，３组ＤＮＡ倍性＞５Ｃ细胞为１．２５％，４组为５．０％。两组间存在极显著差异（Ｐ＜０．０１）．表明形态计量及ＤＮＡ含量测定对实验性肺腺癌研究有较大的实用价值。A computer-assisted image analysis system was applied to quantitate the cellular morphometry and DNA content of normal epithelia of bronchi (Group 1),hyperplastic epithelia (Group 2),atypical hyperplastic epithelia (Group 3) and adenocarcinomas (Group 4)in the course of the experimental lung adenocarcinoma induced by non-ferrous metal dust.Among 16 geometrial parameters,7 were chosen for diagnosis and differential diagnosis of lesions of the four groups.Each value was tested by stepwise discrimination classification. the accuracy rate being 96.3%. DNA ploid was investigated, and no case of >5 cell was found in normal and hyperplasis. Severe atypia had a lower percentage (1.25 %) and Lung adenocarcinoma was characterized by a high perecentage of > 5 cell (5.0%). The difference in percentage of > 5 cell between the severe atypia and adenocarcinoma was statistically significant(P<0.01).It is concluded that the cellular morphometry and DNA content measurement is very helpful to the pathological diagnosis of experimental lung adenocarcinoma.

关 键 词：腺癌 图象分析 DNA 肺肿瘤 

分 类 号：R734.203[医药卫生—肿瘤]