人原发性肝癌及癌旁肝组织中Ⅰ、Ⅲ型胶原的基因表达  

Expression of Collagen I and III Genes in Human Primary Liver Cancer

作　　者：王要军[1] 权启镇[1] 戴益民[2] 杨林森[2] 

机构地区：[1]济南军区总医院消化科 [2]第二军医大学病理学教研室

出　　处：《临床肝胆病杂志》1994年第2期73-75,共3页Journal of Clinical Hepatology

摘　　要：用cDNA-mRNA原位核酸杂交技术检测了2例正常人肝及12例原发性肝癌及癌旁肝组织中Ⅰ、Ⅲ型胶原的基因表达,结果发现8例原发性肝细胞癌组织中多种细胞,包括瘤细胞都有胶原基因的表达,证实人原发性肝癌组织中不仅间质细胞而且肿瘤细胞也参与胶原的合成。胶原基因的过度表达可能与细胞的活跃增生有关。With DNA-mRNA in situ hybridization technique, we detected the expression of collagen I and Ⅲ genes in two esses of normal human liver tissue, 12 patients with primary liver cancer and their surrounding liver tissue. The results demonstrated that type I and Ⅲ mRNAs distribution is identical. Type I, Ⅲ genes expression increases in many kinds of cells in primary liver cancer tissues. It was also found that the hepatocellular cancer(HCC)cells have intensive positive signals.It is proved that not only the mesenchymal cells but also tumor cells can produce type I and Ⅲ collagens in human HCC tissue.Active proliferation of the tumor and mesenchymal cells may be associated with the collagens gene overexpression.

关 键 词：肝肿瘤 胶原基因 原位杂交 

分 类 号：R735.703[医药卫生—肿瘤]