苯丙氨酸解氨酶菌株的选育及L─苯丙氨酸的合成  

BREEDING OF STRAIN FOR PHENYLALANINE AMMONIA LYASE AND SYNTHESIS OF L-PHENYLALANINE

作　　者：由英才[1] 邢文革[1] 王补森[1] 王寿亭[1] 何炳林[1] 

机构地区：[1]南开大学化学系,南开大学高分子化学研究所

出　　处：《南开大学学报（自然科学版）》1994年第4期31-36,共6页Acta Scientiarum Naturalium Universitatis Nankaiensis

摘　　要：本文以粘红酵母ＲｈｏｄｏｔｏｒｕｌａｇｌｕｔｉｎｉｓＡＳ２．１０２，１００１－２Ｏ－６Ｒ和深红酵母ＲｈｏｄｏｔｏｒｕｌａｒｕｂｒａＡＳ２．２７９为出发菌株，经紫外诱变筛选得到一株苯丙氨酸解氨酶高活力菌株Ｒｈ．ｇｌｕｔｉｎｉｓＮＣ０６，其酶活力是亲株１００１－２０－６Ｒ的１．５倍。在最适转化条件下，肉桂酸转化率达５７．７％，每升转化液生成Ｌ－苯丙氨酸１１．５４克，另外对ＮＣ０６的生理特性进行了初步研究。Three strain yeasts Rh. glutinis 1001-20-6R. AS2. 102 and Rh rubra AS2. 279 were mutagenised with UV irradiation. NC06,one of the mutants,exhibits 1. 5 times the phenylalanine ammonia lyase activity of parent 1001-20-6R. The optimum conversion condition was 2% the cinnamic acid,8mol/L. NH4OH, pH11. 0, and the reaction temperature was 30℃. Under the optimal conversion condition. L-phenylalanine was 11. 54g/L ,conversion yield for cinnamic acid was 57. 7%. In addition,physiology properties of NC06 were initial study.

关 键 词：菌株 L-苯丙氨酸 苯丙氨酸解氨酶 紫外诱变 

分 类 号：TQ925.9[轻工技术与工程—发酵工程] TS264.9