细小病毒H－1对正常仓鼠肝细胞DNA周期动力学的影响  

EFFECTS OF PARVOVIRUS H-1 ON DYNAMICS OF HEPATIC CELL DNA CYCLE IN NORMAL HAMSTERS

作　　者：杨庆华 罗鸿予 沈冠凤[1] 童菊芳 萧树东[1] 江绍基[1] 

机构地区：[1]上海市消化疾病研究所

出　　处：《上海医学》1994年第4期202-204,共3页Shanghai Medical Journal

摘　　要：健康成年金黄仓鼠腹腔内接种ＰＶＨ－１（５×１０６ｐｆｕ），ＦＣＭ动态检测肝细胞ＤＮＡ周期动力学变化，结果显示：ＰＶＨ－１接种后处于Ｇ０＋Ｇ１期的肝细胞与对照组动物相比无显著差异。Ｓ期肝细胞在第３天显著高于对照组动物（Ｐ＜０．０５），且在第７天时仍呈上升趋势，第１４天到３０天趋于正常水平。Ｇ２＋Ｍ期肝细胞在ＰＶＨ－１接种后第３０天升高（Ｐ＜０．０５）。结果提示，动物在ＰＶＨ－１接种后第３到第７天是病毒在肝细胞中的复制高峰，并阻滞仓鼠处于Ｓ期肝细胞分裂进入Ｇ２＋Ｍ期，但是这种阻滞作用较短暂、并可逆，在ＰＶＨ－１接种后１４～３０天自然消失。he normal healthy golden hamsters were inoculated parvovirus H-1,PVH-1(5×106pfu)intraperitonially and changes of cell DNA cycle were studied by flow cytometry.The results showed that there were no significant difference between treatment and controlgroup in cell G0+G1 phase after PVH-1 injection.However there was significant incre-ase of S phase hepatic cells on the third day after injection (P<0.05),and it kept athigh level persisting for 4 days,since then,it returned to the basal level,There wasslight increase of G2+M phase hepatic cells 30 days after injection of PVH-1.It is suggested that virus replication peak time was on the third to seventh dayafter PVH-1 injection and virus replication could interrupt the S phase of hepatic cellsdeveloping into G2+M phase.This abnormality,however,was reversible and disappeared14～30 days after PVH-1 inoculation.

关 键 词：细小病毒H-1 DNA周期 流式细胞仪 

分 类 号：R730.51[医药卫生—肿瘤]