土壤农杆菌转化的长春花冠瘿细胞培养  

Study on Cell Suspension Culture of Catharanthus roseus Crown Gall Cell Induced by Agrobacterium C_(58)

作　　者：王宁宁[1] 王淑芳[1] 田俊英[1] 李霞 朱亮基[1] 

机构地区：[1]南开大学生物化学及分子生物学系,天津300071

出　　处：《生物工程学报》1994年第3期244-249,共6页Chinese Journal of Biotechnology

摘　　要：以土壤农杆菌C_(58)诱导的长春花冠瘿组织与从长春花茎、叶外植体诱导的愈伤组织进行比较,发现冠瘿组织在生长、总吲哚生物碱含量及药用成份阿吗碱含量等方面都优于愈伤组织。测定了光照、温度、蔗糖浓度及外加L-色氨酸前体等,对长春花冠瘿细胞的生长、总生物碱及阿吗碱含量的影响,为长春花冠瘿细胞培养生产吲哚生物碱的实际应用研究提供理论依据。In comparison with calli from C. roseus leaf or stem, C. roseus crown gall cell cultured on MS basic medium was superior in growth, total indole alkaloids and aj-malicine contents. Several cultural factors can affect production of alkaloids by C. roseus crown gall cell cultures. When illuminating-cultured or cultured in the control temperature (25℃), the contents of total alkaloids and ajmaficine were higher than those cultured in continuous dark or lower temperature. Treating with exogenous L-Trp or increasing sucrose level of medium can greatly improve the total alkaloids and ajmalicine contents of C. roseus crown gall cell cultures. The most affective concentration of exogenous L-Trp for production of total alkaloids and ajmalicine were 900mg/L and 700mg/ L, respectively. The most effective concentration of sucrose was 80g/L. These results imply that C. roseus crown gall cell be used in replacing calli cells to producing useful indole alkaloids.

关 键 词：长春花 冠瘿组织 细胞工程 

分 类 号：R394.8[医药卫生—医学遗传学]