离体缺血再灌注鼠心肌钙离子的变化  

The Change of Ca￣2+ in SR and Mit of IsolatedIschemnia-Reperfusion Rat Heart.

作　　者：车成日[1] 李香善[1] 金基焕[1] 王明勇[1] 李素香[1] 李丹[1] 

机构地区：[1]延边医学院附属医院胸心外科,延边医学院心血管研究室

出　　处：《生物化学与生物物理进展》1994年第1期73-76,共4页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金

摘　　要：用液体闪烁计数法测定离体再灌注鼠心肌肌质网（ＳＲ）和线粒体（Ｍｉｔ）内￣（４５）Ｃａ￣（２＋）放射性强度（ｃｐｍ），比较能量制剂ＡＴＰ－ＭｇＣｌ＿２，活性氧自由基清除剂ＳＯＤ和钙阻滞剂Ｖｅｒａｐａｍｉｌ对离体缺血再灌注鼠心肌细胞ＳＲ和Ｍｉｔ钙离子浓度的影响。结果表明，ＳＲ内ＡＴｐ－ＭｇＣｌ＿２，ＳＯＤ和Ｖｅｒａｐａｍｉｌ组￣（４５）Ｃａ￣（２＋）的ｃｐｍ均高于对照组（Ｐ＜０．０ｌ或Ｐ＜０．０５），而Ｍｉｔ内均低于对照组（Ｐ＜０．０１）．此三种药均能提高离体大鼠心肌细胞内ＳＲ￣（４５）Ｃａ￣（２＋）和降低Ｍｉｔ￣（４５）Ｃａ￣（２＋）积聚，从而保护了心肌细胞，防止缺血再灌注损伤。he strength of radiation of ￣45Ca￣2+ in sar-coplasmic reti6ulum vesicle (SR) and mito-chondria (Mit) was measured by liguid scintil-lation counting method. Comparing the effectsof ATP - MgCl_2 . SOD and verapamil on the￣45Ca￣2+concentration in SR and Mit which pre-pared from isolated ischemia reperfused rathearts. The result showed that the ￣45Ca￣2+ cpmof SR of the three groups were higher thanthat of control, while the ￣45Ca￣2+cpm of Mit ofthe three groups were lower than that of con-trol. These results suggested that the threekinds of reagent could protect reperfusion in-jury of heart cell through enhance ￣45Ca￣2+ stor-age in SR and inhibitting the ￣45Ca￣2+ accumu-lation in Mit.

关 键 词：钙离子 缺血 再灌注损伤 心肌 

分 类 号：R362[医药卫生—病理学]