酰化菌紫质的动力学光谱及光电特性研究  

Studies on tbe Photoelectric and Kinetic Spec-troscopic Properties of Acetylation Bacteri-orhodopein.

作　　者：胡坤生[1] 施桦[1] 黄莹[1] 余福[1] 

机构地区：[1]中国科学院生物物理研究所,青岛医学院,浙江大学光学实验仪器工程系

出　　处：《生物化学与生物物理进展》1994年第2期150-153,共4页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金;浙江省自然科学基金

摘　　要：用人工双分子膜（ＢＬＭ）技术及动力学光谱研究了赖氨酸残基在紫膜的结构和功能中所起的作用。酰化基团与菌紫质（ｂＲ）分子中的赖氨酸残基的ε氨基作用，使光照后ｂＲ的跨膜质子迁移信号及膜的充放电速度变慢，光循环中间产物Ｍ４１２的产量下降，半衰期延长。但ＵＶ／Ｖｉｓ吸收光谱表明酰化并未破坏ｂＲ中视黄醛的构象环境。在高ｐＨ或高盐浓度下，酰化的影响降低。这些结果表明：赖氨酸残基并不是泵出质子的提供者，没有直接参与质子的跨膜输运，而是通过表面电位来影响ｂＲ的质子泵功能。he role of lvsine residues in the structure andfunction of bacteriorhodopsin(bR) was stud-ied by the chemical modification method──acetylation. After acetylation, the photore-sponse signals and the decay of photocycle in-termediate M412 were slowed down while theyields of M412 were decreased. But UV/VISabsorption spectra did not show that the con-formation around retinal chromophore was dis-turbed by acetylation. The effect of acetylationwas weakened by high PH or salt media. Theresults imply that lysine residues do not direct-ly participate in the proton translocation, in-stead, they affect this process by their contri-bution to the surface protentials.

关 键 词：菌紫质 光电响应 紫膜 光谱 

分 类 号：Q734[生物学—分子生物学]