酵母PHO81蛋白的结构预测和功能分析  

Conformation predication and Function Analysis of Yeast PHO81 Protein

作　　者：吴健生[1] 敖世洲[1] 

机构地区：[1]中国科学院上海生物化学研究所

出　　处：《生物化学与生物物理学报》1994年第3期283-287,共5页

摘　　要：以Ｃｈｏｕ－Ｆａｓｍａｎ和ＧＯＲ方法预测酵母ＰＢＯ８１蛋白的二级结构。用Ｋｙｔｅ－Ｄｏｏｌｉｔｔｌｅ方法分析它的疏水性。分析了ＰＨＯ８１蛋白与其他蛋白的同源性，发现它包含５个ＳＷＩ６／ＡＮＫ重复单位，这些重复单位可能是ＰＨＯ８１蛋白与负调控因子ＰＨＯ８０相互作用的位点．Using the Chou-Fasmanand GOR methods,we have Predicated the secondarystructure of the deduced amino acid sequences of Previously cloned yeast PHO81 gene. Its hydrophobicity has also been determined by the Kate & Doolittle method. While comparing its amino acid sequences with other Proteins, we find that the PHO81 Protein contains five SW16/ANK repeats, which are ubiquitous motifs involved in macromoleoular recognition. These repeats may serve as recognition sites of PHO81 Protein with PHO80, which is a negtive factor in the regulation system of yeast acid phosphatase.

关 键 词：酵母 蛋白 结构 预测 功能 

分 类 号：Q939.506[生物学—微生物学]