肝癌组织中GD3合成酶的性质研究  

Characterization of GD3 Synthetase from Rat Liver Cancer

作　　者：谢天培[1] 夏霞娟[1] 顾天爵[1] 

机构地区：[1]上海医科大学基础医学院生物化学教研室

出　　处：《生物化学与生物物理学报》1994年第4期437-440,共4页

基　　金：国家自然科学基金

摘　　要：肝癌组织中ＧＤ３合成酶的性质研究谢天培，夏霞娟，顾天爵（上海医科大学基础医学院生物化学教研室，２０００３２）关键词肝癌；ＧＤ３合成酶；特性我们先前的研究发现肝癌组织中神经节着脂ＧＤ３的含量增加，并证明这与ＧＤ３合成酶（ＯＭＰ－－ＮｃｌｌＡＩ：ＧＭ３唾...The characterization of GD3 synthetase showed that its optimum PH was 6.25,apparent Km. values for CMP-SA and GM3 were 18.8 and 10.5 μM respectively.The enzyme was found to be stimulated by Mn2+,Mg2+,cholesterol,cardiolipin, sphingomyelin, L-PC, DL-PE, L-PS,L-PA;and inhibited by Cu2+,Zn2+,CTP,CDP, L-PI,L-PE 2,3-DPG,Diolein. It is of interest that phorbol ester(TPA)can strongly stimulate the enzyme activity and the possible mechanism involving hydrophobic interaction between TPA and the enzyme was considered. It was also shown that the enzyme activity was inhibited by protein kinase C,possibly due to its Phosphorylation.

关 键 词：肝肿瘤 GD3合成酶 特性 

分 类 号：R735.702[医药卫生—肿瘤]