肝癌组织中GD3合成酶的纯化  

Purification of GD3 Synthetase from Rat Liver Cancer

作　　者：夏霞娟[1] 谢天培[1] 顾天爵[1] 

机构地区：[1]上海医科大学基础医学院生物化学教研室

出　　处：《生物化学与生物物理学报》1994年第4期351-356,共6页

基　　金：国家自然科学基金

摘　　要：ＧＤ３合成酶（ＣＭＰ－ＮｅｕＡｃ：ＧＭ３唾液酸转移酶，ＳＴ２）是膜嵌合蛋白，定位于高尔基体。经过制作微粒体、Ｔｒｉｔｏｎ增溶、ＡＨ－Ｓｅｐｈａｒｏｓｅ及ＧＭ３－Ｇｌａｓｓｂｅａｄｓ亲和层析等步骤，二乙基亚硝胺诱发大鼠肝癌组织中的ＳＴ２被纯化了３１５９７倍，得率为０．３５％。ＳＤＳ－聚丙烯酰胺凝胶电泳后银染色呈１条蛋白着色带，分子量为５５ｋｄ．GD3 synthetase(ST2)is a membrane protein localized in the Golgi body.This paper reported its purification to homogeneity on SDS-PAGE. Liver cancer tissue from diethylnitrosamine-induced rat was homogenized and centrifuged.The membrane precipitation was solubilized with 1% Triton X-100/Triton CF-54(W/W1:1). After aminohexyl-Sepharose and GM3-Glassbeads affinity chromatography, the enzyme was purified by 31597 times. Its yield was 0.35%.Thepurified product gave one band on SDS-PAGE with silver staining with a MW of55kd.

关 键 词：GD3 合成酶 肝肿瘤 神经节苷酯 

分 类 号：R735.702[医药卫生—肿瘤]