二甲基苯蒽对小鼠皮肤表皮鸟氨酸脱羧酶和转化生长因子βmRNA水平的影响  

Effects of 7,12-Dimethylbenz[a] Anthracene on the Levels of Ornithine Decarboxylase and Transforming Growth Factor-β mRNA in Mouse Epidermis

作　　者：孙士勇[1] 张乃蘅 

机构地区：[1]北京医科大学生化教研室,北京医科大学天然药物及仿生药物国家重点实验室

出　　处：《生物化学杂志》1994年第1期21-24,共4页

摘　　要：本文利用Ｎｏｒｔｈｅｒｎ印迹分析技术研究了完全致癌物二甲基苯蒽（ＤＭＢＡ）对小鼠表皮鸟氨酸脱羧酶（ＯＤＣ）ｍＲＮＡ和转化生长因子β（ＴＧＦ－β）ｍＲＮＡ水平的影响。ＤＭＢＡ在１５μｇ和９００μｇ剂量下一次涂用于小鼠皮肤，可使表皮ＯＤＣｍＲＮＡ和ＴＧＦ－βｍＲＮＡ表达增加。ＯＤＣｍＲＮＡ为单一的２．０ｋｂ大小的条带，其表达在在３６ｈ时最为明显。而ＴＧＦ－βｍＲＮＡ大小为２．５ｋｂ和１．９ｋｂ，其表达在３６ｈ时最高，到４８ｈ几乎降至正常，但在７２ｈ又有增加。ＯＤＣ和ＴＧＦ－β可能在ＤＭＢＡ诱癌过程中起重要作用。The effects of 7,12-dimethylbena[a] anthracene(DMBA) on the mRNA levels of ornithine decarboxylase(ODC) and transforming growth factor-β (TGF-β) in mouse epidermis were studied by Northern blotting in this paper. A single topical treatment of mouse skin with DMBA at doses of 150μg and 900 μg resulted in increased expressions of ODC and TGF-β mRNA. ODC mRNA was a single major band of 2. 0 kb in size. Its expression appeared to be maximal at 36h after treatment and returned to near control level after 96h.TGF-β mRNA exhibited two bands of 2. 5kb and 1.9kb in size. Its level reached maximum at about 36h and then declined to normal level at 48h. A second, less intense maximum was observed at about 72h after treatment. These results indicate that ODC and TG-βmay play roles in DMBA-induced carcinogenesis.

关 键 词：二甲基苯蒽 致癌物 鸟氨酸脱羧酶 

分 类 号：R730.231.1[医药卫生—肿瘤]