R－藻红蛋白──金（AUC4）和汞（PCMS）重原子衍生物的制备和分析  

PREPARATION OF HEAVY ATOM DERIVATIVES OF R-PHYCOERYTHRIN CRYSTAL FROM POLYSIPHONIA URCEOLATA

作　　者：万柱礼[1] 江涛[1] 常文瑞[1] 宋海卫[1] 张季平[1] 桂璐璐[1] 汪曙光[1] 梁栋材[1] 

机构地区：[1]中国科学院生物物理研究所,生物大分子国家重点实验室

出　　处：《生物物理学报》1994年第4期552-556,共5页Acta Biophysica Sinica

基　　金：中国科学院重大项目基金

摘　　要：在获得适合Ｘ射线衍射分析用的Ｒ－藻红蛋白单晶体的基础上，用重原子浸泡法，将晶体浸入含重金属金和汞的０．０５ｍｏｌ／Ｌ磷酸钠－硫酸铵的母液内，经对晶体衍射点强度的分析．找出有明显变化的重原子衍生物。最终得到了与Ｒ－藻红蛋白晶体同晶型的含金和汞的两种重原子衍生物。用面探测仪分别收集了这两种重原子衍生物的衍射数据。通过差值Ｐａｔｔｅｒｓｏｎ图分析，分别确定了重金属金和汞的位置，经对重原子位置参数精化后，给出的品质因子结果表明，含金和汞的重原子衍生物均可被用于多对同晶置换法求解Ｒ－藻红蛋白晶体母体相角的计算。On the basis of growing single Crystals suitable for X-ray analysis, two heary atom derivatives of R-Phycoerythin(R-PE) have been obtained in 0.05mol/L sodium phosphate buffer and (NH4)2SO4 system containing K2AuCl4 and P-chloromercuriphyl sulphonic acid reagents by soaking native crystals method. Changes in X-ray diffraction pattern using Buerger's processing photograph were found. The data of native and both derivatives of R-PE crystals were collected on Siemens X-200B area detector diffractometor. The positions of gold and mercury atoms were determined by using Patterson difference analysis. The Figure of merit of both heavy atom parameters showed that native phases can be obtained by the multiple isomorphous replacement method.

关 键 词：藻类 R-藻红蛋白 重原子衍生物 单晶体 金 汞 

分 类 号：Q949.207[生物学—植物学] Q71