四膜虫(Tetrahymena thermophila)核纤层的研究  

INVESTIGATION OF NUCLEAR LAMINA IN TETRAHY- MENA THERMOPHILA

作　　者：陈彬[1] 蔡树涛[1] 翟中和 

机构地区：[1]北京大学生物学系,北京100871

出　　处：《实验生物学报》1994年第2期152-163,共12页Acta Biologiae Experimentalis Sinica

基　　金：国家自然科学基金

摘　　要：采用非树脂包进埋去包埋超薄切片电镜技术,并结合选择性生化抽提方法以及间接免疫荧光染色方法显示,在四膜虫(Tetrahymena thermophila)细胞内存在典型的核纤层结构。蛋白分离纯化与免疫印迹法分析结果说明四膜虫细胞核纤层可能主要由分子量为66KD的多肽构成。蛋白分离纯化结果表明四膜虫细胞的核纤层蛋白可能不如高等动物细胞的丰富。The nuclear lamina in the nuclei of Tetrahymena thermophila was investigated using the techniques of selective extraction and embedment-free electron microscopy, as well as immunofluorescence staining. By means of immunoblotting and protein isolating, the lamina was found to consist mainly of a 66 KD polypeptide. While rabbit-anti-lamins polyclonal antibodies crossreacted with this protein, neither mu-rine-anti-lamin A&C mAB C 23 nor murine-anti-lamin 1 mAB 223 showed positive reaction with it. It was also demonstrated that the lamin is not so rich as that in mammalian cells. The existence of lamina in T. thermophila, a unicellular organism, seems to show that the lamina is a basic characteristic structure of eu-karyotic cells, and that the function of the lamina may not be limited to the process of breakdown and reconstitution of nuclear envelope during mitosis.

关 键 词：四膜虫 核纤层 蛋白 鞭毛纲 

分 类 号：Q959.117.3[生物学—动物学]