杜氏利什曼原虫人工感染家犬血清中IgG动态观察  

IgG LEVELS IN THE SERA OF DOGS EXPERIMENTALLY INFECTED WITH L DONOVANI PROMASTIGOTES

作　　者：刘佩娜[1] 王子龙[1] 阚兵[1] 林芳清[1] 

机构地区：[1]华西医科大学寄生虫学研究室

出　　处：《实用寄生虫病杂志》1994年第4期32-34,共3页Journal of Practical Parasitic Diseases

基　　金：华西医科大学校科学基金

摘　　要：本文采用杜氏利什曼原虫四川人分离株前出毛体人工感染家犬１１只，按不同感染剂量（１×１０４～１×１０８／只）分组并设未感染对照犬２只，骨髓涂片法感染组均查见利什曼原虫无鞭毛体，感染成功。感染前犬血清ＩｇＧ含量均数为１２６．５９Ｉｕ／ｍｌ，１５周时达１９２．５４Ｉｕ／ｍｌ，（Ｐ＜０．０５）。说明本试验人工感染Ｌ．ｄ前毛体导致了犬ＩｇＧ的上升。This study reports that 11 dogs were experimentally infected by pcritoneal inoculation of L. donovani Sichlian human isolates (MGOM/CN /90/SCW12 ) ptomastigotes ranged, frin 1×104to 1×108, and 2 dogs were served as controls. After being infected for about 9 weeks, 6 dogs were positive by examillation for the existance of amastigotes by bone marrow smear method.After being infected for 22 weeks, 90. 9% dogs were successfully inflected. At the tirnc beforeinoculation, post' inoculation of 2, 4, 8, 12, 15 and ZI weeks, all dogs. scra were examined for determining the levl of IgG using Beck man immunochemistry immunochen,istry systeTns analyzer 11. respectively.The mean level of. IgG pre infection was 126. 59 In/ml. 4 and fo weeks after infection their lgGlevels became:188. 11 and 192. 54 In/ml respectively. There ic statistical significance bet'veenpred and post-infection (P<0. 05).

关 键 词：杜氏利什曼原虫 无鞭毛体 前鞭毛体 犬 IGG 

分 类 号：R382.22[医药卫生—医学寄生虫学]