小儿ALL细胞极限稀释液体微培养及微小残留病的检测  

Micro-liquid colony culture of childhood acute lymphoblastic leukemia cells by limited-dilution assay and detection of minimal residual disease

作　　者：董勤[1] 郭淑芬[1] 徐淑芝[1] 余钟声[1] 

机构地区：[1]浙江医科大学附属儿童医院

出　　处：《实用肿瘤杂志》1994年第2期79-82,共4页Journal of Practical Oncology

基　　金：浙江省卫生厅课题资助

摘　　要：首次用ＣＦＵ－ＡＬＬ极限稀释液体微培养法，对１６例初诊，１４例ＣＲ期ＡＬＬ骨髓进行培养，并用８例正常，１１例再生骨髓作为ＣＦＵ－ＡＬＬ阴性对照。结果显示：初诊Ｂ系ＡＬＬ骨髓以重叠型集落伴ＣＤ（１０＋）细胞＞２０％为特点；初诊Ｔ－ＡＬＬ集落也属重叠型（ＣＤ（２＋）、ＣＤ（３＋）、ＣＤ（７＋））．而正常和再生骨髓虽形成集落，但为平展型或伴重叠型集落（ＣＤ（１０＋）＜２０％、以ＣＤ（１５＋）、ＣＤ（３３＋）细胞为主）。本文１４例ＣＲ期ＡＬＬ中，７例有重叠型集落（ＣＤ（１０＋）细胞＞２０％）；临床随访１年半，５例骨髓相继复发，１例诊断为睾丸白血病。The cells of bone marrows from patients with childhood ALL(newly diagnosed:16 cases;in remission:14 cases)were culured undetr low O2 tension by means of micro-liquid CFU-ALL limited-dilution assay.Eight normal and eleven regenerating bone marrows were used as negative controls of CFU-ALL.The characters of colony cells were defined on the basis of colony morphologic appearance and colony cell-surface markers.The results showed:the newly diagnosed B-lineage ALL bone marrows yielded overlapping colonies with CD10+ cells>20%.The newly diagnosed T-ALL bone marrows also formed overlapping colonies(CD2+ CD5+ CD7+ cells).The normal and regenerating bone marrows also yielded colonies(flat or with overlapping colonies) under similar cultural conditions,but the number of CD10+ colony cells was low(<20%).The colonies mainly consisted of CD15+ CD33+ cells.On the basis of the characters of B lineage ALL colonies,the bone marrows of 7 patients with ALL in remission were detected to have overlapping colonies with CD10+ cells>20%,which was suspected to be the sign of residnal B-lineage ALL cells.These patients were followed up.The authors consider the assay will be valuable in studies on leukemia in vitro and detection of minimal residual disease.

关 键 词：白血病 液体微量培养 微小残留病 

分 类 号：R733.710.3[医药卫生—肿瘤]