胡芦巴水溶性甾体皂苷的提取分离工艺研究  

Studies on Extracting Technology of Water-soluble Steroidal Saponins from Fenugreek Seeds

作　　者：张黎明[1] 徐玮[1] 杜连祥[1] 

机构地区：[1]天津科技大学生物工程学院,天津300222

出　　处：《天然产物研究与开发》2005年第1期47-50,共4页Natural Product Research and Development

基　　金：天津市高等学校科技发展基金项目 (2 0 0 3 12 10 )

摘　　要：以薯蓣皂苷元的提取率为指标 ,应用L9(34)正交试验设计优化了从脱多糖、脱脂胡芦巴豆粉中提取甾体皂苷的工艺条件 ,并且筛选了ZTC澄清剂对胡芦巴甾体皂苷水提取液的澄清条件。实验结果表明 ,影响水提取的主次因素为 :提取次数 >提取温度 >固液比 >提取时间 ;最佳水提取条件为 :固液比为 1:10 ,在 70℃下提取 3次 ,每次 12. 0min。得出胡芦巴种子水提取液的澄清条件如下 :ZTC1+1澄清剂的加入次序是先加B组分再加入A组分 ;澄清剂B和A的最佳用量分别为 1 .0g/L和 0 . 5g/L ;加入组分A或B后在 80℃下作用 30min即可达到澄清目的。Using the remainder of fenugreek seeds with separated endosperm as the raw material,the optimum extraction conditions of steroidal saponins were investigated by orthogonal design and with the extracting rate of diosgenin as index.The clarifying conditions of steroidal saponins aqueous solution was also selected by using ZTC flocculating method.The results show that the factors influencing extraction efficiency were as follows:extraction times > temperature> ratio of feeding stock weight to the volume of solvent> duration of extraction.The optimum extraction condition obtained was:adding 10 times of water volume as much as that of sample and extracting for 120 min at 70 ℃(totally extracting for 3 times).The adding sequence of ZTC1+1 natural clarifying agent was clarifier B before clarifier A.The optimum amount of ZTC clarifier was 1.0 g/L for clarifier B,and 0.5 g/L for clarifier A.The clarifying time after adding clarifier B and A was 30 min.Under these conditions,the steroidal saponins aqueous solution would become clarify and stability.

关 键 词：甾体皂苷 胡芦巴 水提取液 提取分离 薯蓣皂苷元 澄清剂 巴豆 固液比 豆粉 提取率 

分 类 号：Q949.751.9[生物学—植物学] R284[医药卫生—中药学]