RP-HPLC法测定生化汤单煎液与共煎液中阿魏酸的含量  

Determination of Ferulic Acid in Shenghuatang Decocted Together and Decocted Separately by RP-HPLC

作　　者：王雁梅[1] 史恒军[1] 甘洪全[2] 王保秀[3] 沈彤[2] 

机构地区：[1]第四军医大学唐都医院,陕西西安710038 [2]第四军医大学基础部药理教研室,陕西西安710032 [3]浙江医药高等专科学校,浙江宁波315010

出　　处：《中国中医药信息杂志》2005年第3期47-48,共2页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：陕西省自然科学基金资助项目(2001SM43)

摘　　要：目的比较生化汤单煎液与共煎液中阿魏酸的含量。方法采用反相高效液相色谱法,InertsilODS-3C18柱(250mm×4.6mm,5μm);甲醇-水(含1%乙酸)(45∶55,v/v)为流动相;流速1.0mL/min;检测波长为321nm;外标法定量。结果阿魏酸在0.6～180.0mg/L范围内与峰面积呈良好的线性关系(r=0.9999,n=6),平均回收率为102.65%。结论共煎液中阿魏酸的含量明显高于单煎液(P<0.01)。Objective To compare the contents of ferulic acid in Shenghuatang decocted together and decocted separately. Methods The determination was carried on RP-HPLC. Using Inertsil ODS-3 C18 column (250 mm×4.6 mm, 5 μm), the mobile phase was methanol-water (containing 1% acetic acid) (45∶55, v/v) with flow rate of 1 mL/min and at the detection wavelength of 321 nm, and the contents were calculated by external-standard method. Results The calibration curve was linear in the range of 0.6～180.0 mg/L (r =0.9999, n =6). The average recovery was 102.65%. Conclusion The content of ferulic acid in Shenghuatang decocted together was higher than that in Shenghuatang decocted separately (P<0.01).

关 键 词：生化汤 单煎 共煎 阿魏酸 反相高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]