姜黄素对NB4细胞的增殖抑制作用及其作用机制  

Anti-proliferation effect of curcumin on NB4 cells and its mechanism

作　　者：刘加军[1] 黄仁魏[1] 林东军[1] 林曲[1] 李铭权[1] 吴祥元[1] 潘祥林[2] 彭军[2] 

机构地区：[1]中山大学附属第三医院血液科,广东广州510630 [2]山东大学医学院血液肿瘤中心,山东济南250012

出　　处：《中国新药与临床杂志》2005年第3期173-176,共4页Chinese Journal of New Drugs and Clinical Remedies

摘　　要：目的:探讨姜黄素对急性早幼粒细胞白血病细胞株NB4细胞的增殖抑制作用及其作用机制.方法:以0,5,10,20,30 μmol·L-1浓度的姜黄素作用于体外培养的NB4细胞,分别于培养后12,24,36,48,72,72 h用四氮唑蓝比色法检测细胞生长抑制率,应用流式细胞仪及Hoechst 33258荧光染色法观察细胞凋亡,采用聚合酶链反应-酶标记免疫吸附法检测细胞凋亡前后的端粒酶活性.结果:10～30 mol·L-1浓度的姜黄素作用24～72 h可显著降低NB4细胞端粒酶活性,抑制细胞的生长,诱导细胞发生凋亡.姜黄素对细胞的增殖抑制作用呈现出一定的剂量-效应与时间-效应关系,细胞凋亡率在药物作用60h左右达高峰.结论:姜黄素具有显著的体外抗白血病作用,降低细胞端粒酶活性可能是其重要作用机制之一.AIM: To investigate the anti-proliferation effect of curcumin on acute promyelocytic leukemia NB4 cells and its mechanism. METHODS: NB4 cells in vitro were incubated in the culture medium of 0,5,10,20 and 30 μmol·L -1 concentrations of curcumin. The inhibitory rate of the cells was measured by microculture tetrazolium (MTT) assay at 12,24,36,48,60 and 72 h. Apoptosis was observed by flow cytometry (FCM) and Hoechst 33258 fluorescence staining, and the activity of telomerase was detected by telomere repeat amplification protocol-polymerase chain reaction-enzyme-linked immunosorbent assay (TRAP-PCR-ELISA) before and after occurrence of apoptosis. RESULTS: Curcumin(10- 30 μmol·L -1 ) could decrease the telomerase activity, cause apoptosis and inhibit the growth of NB4 cellssignificantly after 24-72 h of incubation. The suppression effect was both on time- and dose-dependent manner, and the apoptotic rate of curcumin treated cells arrived its peak at 60 h. CONCLUSION: Curcumin has apparent anti-leukemia effect in vitro with decreasing the telomerase activity of NB4 cells as one of the most important mechanisms.

关 键 词：姜黄素 白血病 端粒 末端转移酶 细胞凋亡 

分 类 号：R733.7[医药卫生—肿瘤] R979.1[医药卫生—临床医学]