甲基莲心碱对脂质过氧化和活性氧自由基的作用  

The Effects of Neferine on Lipid Peroxides and Active Oxygen Free Radical

作　　者：贾菊芳[1] 敖明章[1] 胡本容[1] 李勇军[1] 郭毛娣[2] 莫长耕[3] 

机构地区：[1]同济医科大学基础医学院药理学教研室,武汉430030 [2]中国科学院抗菌素研究所,北京100050 [3]中国预防医学科学院职业病研究所,北京100050

出　　处：《同济医科大学学报》1994年第1期62-64,共3页Acta Universitatis Medicinae Tongji

摘　　要：用硫代巴比妥酸（TBA）比色法、电子自旋捕集技术、化学发光法及检测苯甲酸──OH．加成物等方法，研究了甲基莲心碱（Nef）清除氧自由基及抗脂质过氧化作用。Nef0.1～5.0μmol／L剂量依赖性地抑制小鼠肝匀浆温孵引起的丙二醛（MDA）升高；Nefl.0μmol／L也有效抑制由乙酸豆寇佛波醇刺激多形核粒细胞产生的化学发光。用核黄素光照法测O_2^-，Nef93.3μmol／L及超氧化物歧化酶10~6U／L对O_2^-的清除率分别为73.6％及72.9％，而对OH的清除效能则为羟自由基清除剂甘露醇的106倍。结果表明，Nef是一有效的植物来源的抗氧化剂。By using thiobarbituric acid reaction,spin trapping technique,chemiluminescence method,fluorescence assay of benzoic acid OH-adducts etc,the effects of Nef on lipid peroxides and O^- or OH in free radical system were studied.Nef 0.1-5.0μmol/L de creased dose-dependently the formation of MDA induced by incubating mice liver ho mogenate at 37℃ for 30 min;Nef 1.0μmol/L also effectively inhibited chemilumlnescence of PMN stimulated by PMA.Nef was found to be able to scavenge and inhibit O_2^- and OH free radical,but the latter is stronger than the former.The scavenging rate of O_2^- by adding Ne f 93.3 umol/L and SOD 10~6 U/L were 73.6% and 72.9% respectively,but the scavenging po tency of the effect on OH free radical is 106 times stronger than mannitol.The results sug gest that Nef is an effective antioxidant of plant origin.

关 键 词：甲基莲心碱 氧 自由基 过氧化脂质 

分 类 号：R282.710.5[医药卫生—中药学]