银耳的超声波介导转化  

Transformation of Tremella fuciformis by Using Ultrasonic Mediation

作　　者：谢宝贵[1] 饶永斌[1] 郑金贵 

机构地区：[1]福建农林大学生命科学学院 [2]福建农林大学农产品品质研究所,福州350002

出　　处：《农业生物技术学报》2005年第1期42-45,共4页Journal of Agricultural Biotechnology

基　　金：福建省科技项目(No.2001Z010)基金资助

摘　　要：以银耳(Tremellafuciformis)芽孢为实验材料,正交实验研究超声波处理时间、间歇时间及功率对转化率的影响。实验结果表明:超声波处理时间与间歇时间对质粒DNA和芽孢转化率都有显著影响;实验中质粒DNA转化率最高达到754.7个转化子/μgDNA,银耳芽孢转化率达到1.28‰;转化子的潮霉素抗性可达到200μg/mL,GUS检测阳性。Yeast-like conidia of Tremella fuciformis was used as material to study ultrasonic mediated transformation. The effect of ultrasonic treatment time, intermission and power on transformation rate was studied with orthogonal experiment. The results showed that ultrasonic treatment time and intermission had a significant influence on the transformation rates of both plasmid DNA and conidia; the highest transformation rates of plasmid DNA and conidia cell were 754.7 transformants /μg DNA and 1.28‰, respectively; The hygromycin resistance of transformants reached 200 μg/mL and the GUS activity was tested positive.

关 键 词：介导转化 银耳 潮霉素 芽孢 抗性 转化率 超声波处理 质粒DNA 阳性 影响 

分 类 号：S436.421[农业科学—农业昆虫与害虫防治] S572[农业科学—植物保护]