人食管癌细胞系Eca109在抗癌药物筛选试验中的应用  

APPLICATION OF Eca109 HUMAN ESOPHAGEAL CANCER CELL LINE TO IN VITRO SCREENING ASSAY OF ANTICANCER DRUG

作　　者：臧梦维[1] 平庆功[1] 董伟华[1] 孔天翰[1] 钱玉珍[1] 刘桂亭[1] 

机构地区：[1]附属弋矶山医院干内科,河南医科大学病理生理学教研室

出　　处：《皖南医学院学报》1994年第3期192-195,共4页Journal of Wannan Medical College

摘　　要：用人食管癌细胞株（Ｅｃａ１０９）进行了丝裂霉素（ＭｉｔｏｍｙｃｉｎＣ，ＭＭＣ）抗癌药物敏感性研究。结果表明，与对照组相比，ＭＭＣ浓度为０．２５μｇ／ｍｌ时，其细胞生长抑制率为６１．９６％（Ｐ＜０．０１）；集落形成抑制率为６４．４６％（Ｐ＜０．０１）．ＭＴＴ显色法证实，ＭＭＣ引起的细胞毒性与药物作用时间成正相关（ｒ＝０．９９９６，Ｐ＜０．０１），ＭＭＣ可抑制细胞的增殖速度，还使癌细胞的有丝分裂指数降低，提示ＭＭＣ对Ｅｃａ１０９细胞呈毒性作用，该细胞株保持了人体食管癌的药物敏感性，故可用于体外筛选新抗癌药。ln this paper, the chemosensitivity of anticancer drug, Mitomycin C(MMC), was investigated by using a human esophageal cancer cell line(Eca109).The result showed that compared with the control,the Eca109 cell growth-inhibited rate induced by MMC at the concentration of 0.25μg/ ml was 61.96% in tyrpan blue exclusion test (P <0.01), and the inhibitory rate of colony formation of MMC at the samcconccntration was 64.6% in colony-forming assay (P<0.01). By MTT colorimetrie assay,the cytotoxieity of MMC on Eca109 cell was in positive correlationship to the length of the cxposure time(r=0.9996, P<.001).It was found in cell growth curve that MMC inhibited cell prolircration speed.MMC induced the reduction of mitotic index on Eca109 cell.The fact that MMC possessed the cytotoxic effect on Eca 109 cell suggests that the celll line Eca109 may still retain its original drug sensitivity which is consistent with that noted in clinieal esophageal eancer.This cell line may be useful in sereening for new antineoplastic agents.

关 键 词：食管肿瘤 细胞株 药物筛选试验 丝裂霉素 抗癌药 

分 类 号：R735.105[医药卫生—肿瘤] R979.1[医药卫生—临床医学]