检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
作 者:郭悦青[1] 周旭[1] 刘恩宇[1] 卢星榕[1] 易继林[1]
机构地区:[1]华中科技大学同济医学院附属同济医院,湖北武汉430030
出 处:《山东医药》2005年第6期14-16,共3页Shandong Medical Journal
基 金:湖北省科技攻关计划基金资助课题 (No.2 0 0 3 AA3 0 1C43 )
摘 要:目的 研究端粒酶催化亚单位 (h TERT)基因表达在肝细胞肝癌 (HCC)发生发展中的作用及意义。方法 应用免疫组化 (SP法 )和半定量逆转录聚合酶链反应 (RT- PCR)同步检测 6 2例 HCC及其对应癌旁组织、8例正常肝组织中 h TERT及 h TERTm RNA的表达情况 ,分析 h TERT基因表达与 HCC临床病理参数的关系。结果 h TERT蛋白主要定位于肝癌细胞胞浆内 ,偶见核内染色。h TERT和 h TERTm RNA在 HCC中的阳性率分别为 88.71%、82 .2 6 % ,分别明显高于癌旁肝组织的阳性率 9.6 8%、6 .4 5 % ,差异具有非常显著性 (P <0 .0 1)。 h TERT及其 m RNA在 8例正常肝组织均未见表达。统计学分析显示 ,HCC中的 h TERT基因表达与肿瘤分期分级、门静脉有无癌栓、血清中甲胎蛋白表达水平、是否合并 HBV感染等有关 (P <0 .0 5 )。结论 h TERT基因可能在 HCC的发生发展中起着重要作用 ,其表达有望成为 HCC的重要分子生物学指标。Objective To investigate the expression of hTERT gene in human hepatocellular carcinoma(HCC) and to discuss its significance during HCC.Methods Expression of hTERT protein in HCC and the paired para-carcinoma liver tissues from 62 HCC patients and in 8 normal liver tissues was detected by immunohistochemistry(SP method) and hTERT mRNA expression was analyzed by reverse transcription polymerase chain reaction(RT-PCR).Results Expression of hTERT protein was mainly located in the liver cancer cell plasma,occasionally in cell nucleus.The positive rates of hTERT protein and hTERTmRNA in HCC were 88.71% and 82.26% respectively which were prominently higher than those in the paired para-carcinoma liver tissues i.e.9.68% and 6.45% (P<0.01).hTERT protein and hTERTmRNA can not be found in normal liver tissues.hTERT gene expression was correlated with tumor clinical grade and stage,portal cancer embolus,HBsAg(+) and high AFP level (P<0.05).Conclusions hTERT gene expression may play an important role in the genesis and development of HCC.It may serve as an importment molecular-biological indicator in diagnosing and predicating the biological behavior of HCC.
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:216.73.216.249