碘离子对烟草过氧化物酶Ⅰ(TOPⅠ)的荧光猝灭的研究  

The Research on the Quenching of Fluorescence Spectra of TOPⅠ byIodine Anion

作　　者：夏炳乐[1] 彭敦耕[1] 李敏莉[1] 肖洪 王开保 

机构地区：[1]中国科学技术大学化学系,安徽合肥230026 [2]驻马店卷烟厂,河南驻马店463000 [3]芜湖卷烟厂,安徽芜湖241000

出　　处：《光谱学与光谱分析》2005年第2期239-241,共3页Spectroscopy and Spectral Analysis

基　　金：国家烟草专卖局资金项目(100200101041)资助

摘　　要：通过对碘离子(I-)与烟草过氧化物酶Ⅰ(TOPⅠ)作用前后的荧光光谱的分析,并运用改良的SternVolmer方程处理数据;计算得到I-离子与TOPⅠ上的色氨酸残基的结合位点数及结合常数,讨论了它的荧光猝灭机制和色氨酸残基在TOPⅠ分子中的分布情况。Comparing the fluorescence spectra of tobacco peroxidaseⅠ(TOPⅠ) solution and the solutions titrated by iodine anions, the number of binding locus and the binding constant of iodine anion to TOPⅠ were calculated by using the modified Stern-Volmer equation. The mechanism of the quenching of fluorescence spectra and the distribution of tryptophane residues in the TOPⅠ molecule were discussed.

关 键 词：碘离子 残基 色氨酸 分布情况 结合位点数 处理 荧光猝灭 烟草 过氧化物酶 改良 

分 类 号：O657[理学—分析化学] R373.9[理学—化学]