培养基对海藻真菌PT2菌株的生长及活性代谢产物合成的影响  

Effects of different kinds of media on the growth and active metabolite production of algicolous marine fungus PT2

作　　者：彭琛[1] 郑忠辉[1] 杜希萍[1] 黄耀坚[1] 宋思扬[1] 苏文金[1] 

机构地区：[1]细胞生物学与肿瘤细胞工程教育部重点实验室,厦门大学生命科学学院,福建厦门361005

出　　处：《台湾海峡》2005年第1期90-96,共7页Journal of Oceanography In Taiwan Strait

基　　金：国家高科技研究发展计划资助项目(2001AA620401);福建省自然科学基金资助项目(C0210001)

摘　　要：本文采用PD、GPY、YES、SYE、GPM 5种不同培养基培养海藻真菌菌株PT2,并对其在不同培养基中的生长情况、代谢产物的形成及抗肿瘤活性进行分析,旨在了解不同培养基对菌株生长和活性物质合成的影响.结果显示,PT2菌株在不同培养基上的培养特征及生长速率有所不同,液体培养基发酵液抽提物的抗肿瘤活性也有较大差别,其中SYE发酵液抽提物活性最强,对KB、Raji和Hela　3种肿瘤细胞的IC50分别为 7. 6μg/cm3、2. 4μg/cm3和 3. 3μg/cm3.采用HPLC法对不同抽提物进行分析,发现它们之间的组成成分有显著差异.本文的实验结果表明,不同培养基对该菌株的生长及活性代谢产物的合成具有显著的影响.Algicolous marine fungus PT2 was cultivated on five different kinds of media which are PD, GPY, YES, SYE and GPM. The growth status, secondary metabolite production of fungus PT2 and anti-tumor activities of extracts from fungus PT2 were analyzed to study the effects of different media on growth and active metabolite production of fungus PT2. The results showed that it was different in culture characters and growth rates of PT2 on different media. There were also various inhibiting effects on tumor cells by five culture broth extracts, the activity of extract from SYE was highest in KB,Raji and Hela tumor cells, IC_(50) was 7.6μg/mm^3, (2.4μg/mm^3) and (3.3μg/mm^3) respectively. Culture extracts of fungus PT2 from five media were determinated by HPLC method and there was significant difference in compositions of extracts. The results indicated that there are distinct effects of different media on growth and active metabolite production of PT2.

关 键 词：微生物学 海藻真菌 生长速率 抗肿瘤活性 HPLC 

分 类 号：Q938.8[生物学—微生物学]