SARS-CoVS蛋白抗原表位多肽的设计合成与免疫反应  

Design synthesis and immue reaction of epitope peptides of SARS-CoV spike protein

作　　者：张嘉杰[1] 吴少瑜[1] 徐伟[1] 吴曙光[1] 

机构地区：[1]中国人民解放军第一军医大学药物研究所,广东广州510515

出　　处：《解放军药学学报》2005年第1期40-42,共3页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：广东省科技厅科研攻关项目SARS专项基金

摘　　要：目的　通过生物信息学与合成多肽方法 ,寻找SARS CoVS蛋白的抗原表位 ,为抗SARS多肽疫苗的深入研究提供试验依据。方法　利用网络共享软件对与S蛋白抗原性相关的参数进行预测分析 ,从而设计S蛋白的抗原表位多肽 ;将合成的抗原表位多肽免疫家兔 ,用ELISA法检测产生的抗体。结果　ELISA法检测发现 ,设计合成的 2个抗原表位多肽片段 (残基 348 380 ,4 34 4 70 )在家兔体内均能诱导抗体产生。结论　设计合成的 2个抗原表位多肽片段中包含SARS CoVS蛋白的抗原表位 ,免疫家兔后能产生特异性抗体。Aim To investigate the epitopes of S protein to develop peptide vaccine against SARS-CoV infection,we designed and synthesized epitope peptides of S protein by combined use of bioinformatical and structural analysis.Methods Epitope peptides of S protein were designed and synthesized after predicting and analyzing the relational parameters of the antigenicity of S protein with online sharing software and rabbits were immunized with these synthetic peptides.Antibodies were tested by ELISA.Results Both of two synthetic peptides (amino acid residues 348-380,434-470) elicited specific antibodies.Conclusion Tow synthetic peptides can elicit specific antibodies in rabbits,which may imply that they contain the epitope sequences of S protein of SARS-CoV.

关 键 词：抗原表位 S蛋白 SARS-COV 合成多肽 免疫反应 家兔 抗SA 残基 利用 试验 

分 类 号：R392[医药卫生—免疫学] R563.1[医药卫生—基础医学]