杀虫双在大鼠体内生物转化的研究  

Biotransformation of Shachongshuang in rats

作　　者：叶菁[1] 王绪卿[1] 赵云峰[1] 朱家琦[1] 

机构地区：[1]中国预防医学科学院营养与食品卫生研究所,深圳市卫生检疫局

出　　处：《卫生研究》1994年第6期352-356,共5页Journal of Hygiene Research

基　　金：国家自然科学基金

摘　　要：建立了大鼠胃、肠中杀虫双和沙蚕毒素的气相色谱定量测定方法。胃和肠中杀虫双平均回收率分别为９７．６％和１１６．２％，沙蚕毒素平均回收率分别为１０２．６％和９９．８％。采用此方法观察经口灌胃杀虫双后在大鼠体内转化成沙蚕毒素的部位以及其转化的动态变化。ＴＬＣ及ＧＣ定性、定量结果均表明胃是主要转化部位。给大鼠灌胃杀虫双（５．０ｍｇ／ｋｇ）１ｍｉｎ后，胃中杀虫双总量（杀虫双及２．３８倍的沙蚕毒素之和）与给药量相当，而此时约３０％杀虫双己转化成沙蚕毒素，给药１ｈ后，胃中未检出杀虫双和沙蚕毒素。杀虫双在胃中消失动态与时间呈良好线性关系。胃中杀虫双浓度与时间的关系为Ｃ＝５５．９８ｅ－０．２５ｔ＋４１．８５ｅ－０．The methods of determination of shachongshuang and nereistoxin in stomach and intestine of rats were established. The average recoveries of Shachongshuang in stomach and intestine were 97.6%and 116.2%respectively and those of nereistoxin were 102.6% and 99.8% respectively.Using these methods,it was shown that the main organ in which Shachongshuang was transformed to nereistoxin was stomach. The toxicokinetic characteristcs following oral intubation with this pesticide(5mg/kg) indicated that after 1 minute the total amount of Shachongshuang(Shachongshuang plus 2. 38 times the amount of Nereistoxin) in the stomach was equal to the amount of intake,and about 30% of Shachongshuang was transformed to Nereistoxin.After 1 hour,neither Shachongshuang nor nereistoxin could be detected in the stomach.The disappearance of Shachongshuanng in stomach showed a linear relationship with time and could be represented by the following equation:C=55. 98e-0.25t+41. 85e-0.06t

关 键 词：杀虫双 沙蚕毒素 大鼠 生物转化 安全性 

分 类 号：R979.8[医药卫生—药品]