蛋白激酶C与K562/A02细胞多药耐药相关性的研究  

The research of the correlation of protein kinase C and the mdr of K562/A02 cells

作　　者：陈宝安[1] 周云[2] 程坚[1] 董颖[1] 钱习军[1] 盛茗[1] 王婷[1] 高峰[1] 

机构地区：[1]东南大学医学院附属中大医院血液科,江苏南京210009 [2]江苏省肿瘤医院,南京210009

出　　处：《白血病．淋巴瘤》2005年第1期7-9,共3页Journal of Leukemia & Lymphoma

基　　金：国家自然科学基金资助项目(39970832)

摘　　要：目的探讨蛋白激酶C(proteinkinaseC,PKC)在K562/A02细胞多药耐药的产生及其逆转中的作用。方法用放射免疫法检测了耐药细胞株K562/A02及其敏感株K562的静息PKC活性水平,并观察了柔红霉素(daunomycin,DNR)以及耐药调节剂粉防己碱(tetrandrine,Tet)、屈洛昔芬(droloxifene,Drol)单独或联合应用后细胞PKC活性的变化。结果静息状态下耐药细胞株K562/A02的PKC活性显著高于敏感株K562,有效调节浓度的Tet、Drol单独作用于K562、K562/A02细胞均可显著下调其PKC活性,联合应用有显著协同性。1μg/mLDNR可显著下调K562细胞的PKC活性,部分下调K562/A02细胞的PKC活性,有效调节浓度的Tet、Drol单独或联合应用均可加强其作用。结论PKC参与K562/A02细胞多药耐药(multidrugresistance,MDR)的形成,Tet、Drol逆转K562/A02细胞的MDR可能与下调其PKC活性有关。Objective To study the effects of protein kinase C(PKC) in the course of the K562/A02 cells'multidrug resistance formation and the effects in it's reverse. Methods Radio-immunity method was used to detect the PKC activity of K562 cells and K562/A02 cells without any stimulation and their PKC activity after cultured with daunomycin, tetrandrine(Tet) and droloxifene(Drol) alone or allied. Results The PKC activity of K562/A02 cells is higher than that of K562 cells significantly. Tetrandrine and Droloxifene in their effective regulating concentration alone can decrease the PKC activity of K562/A02 cells and K562 cells.They have synergism when use together. 1 μg/mL DNR can decrease the PKC activity of K562 cells notablely and decrease the PKC activity of K562/A02 cells partly tetrandrine and droloxifene in their efficient regulating concentration alone or allied all can strenthen this effect of DNR. Conclusions PKC take part in the MDR formation of K562/A02 cells. Tet and Drol reverse the MDR of K562/A02 cells maybe have relation to decrease its PKC activity.

关 键 词：蛋白激酶C 多药耐药 粉防己碱 屈洛昔芬 细胞系 K562 

分 类 号：R73-3[医药卫生—肿瘤]