杨氏欣痨宁胶囊抗结核豚鼠模型的实验研究  

Effect of Yangshixinlaoning capsule on tuberculosis in guinea pig

作　　者：张才军[1] 王玲[1] 章宗籍[2] 钱忠义[2] 谢蒲伶[1] 姚军[1] 杨尚星 

机构地区：[1]昆明医学院微生物学与免疫学教研室,昆明650031 [2]昆明医学院病理学教研室,昆明650031 [3]云南省丽江星盛民族医药研究有限公司,丽江674100

出　　处：《河北中医》2005年第1期62-65,共4页Hebei Journal of Traditional Chinese Medicine

基　　金：云南省教育厅科学研究基金项目;编号 :0 2ZY2 10

摘　　要：目的　通过研究复方中成药制剂杨氏欣痨宁胶囊 (YLN)对豚鼠结核病模型的抗结核作用 ,为YLN的临床前研究提供基础研究依据。方法　动物随机分组 ,建立豚鼠的结核分枝杆菌H 37Rv株感染模型 ,观察以下受试动物指标 :体重、脏器病变程度 (肉眼观察 )、脏器 (脾、肺、肝 )重量指数、豚鼠脏器结构分枝杆菌定量培养的菌落形成单位 (CFU)。结果　①YLN高、中、低剂量组 [1.0 ,0 .5 ,0 .2 5g (kg·d) ]体重显著大于模型空白对照组 ,YLN与异烟肼 (INH)联合用药组豚鼠体重大于INH单用药组 (P <0 .0 5 ) ;②YLN各剂量组肉眼观察脏器病变程度均有改善作用 (P <0 .0 5 ) ;③YLN各剂量组脏器重量指数 ,除YLN低剂量组脾和肝重量指数外 ,其余各组脏器重量指数显著降低 (P <0 .0 5 ) ,INH与YLN联合用药效果优于单用药 ;④YLN高、中剂量组豚鼠脏器结核分枝杆菌定量培养的CFU数显著低于模型空白对照组 (P <0 .0 5 )。结论　各剂量YLN对豚鼠结核模型具有显著抗结核杀菌作用 ,以高、中剂量YLN尤为显著。Objective To observe the effects of Yangshixinlaoning capsule(YLN) on tuberculosis in guinea pig, in order to provide basis of experimental research for the study before being used clinically.Methods Guinea pigs were randomly divided into nine groups. The guinea pigs were injected with the strain of Mycobacterium(M) tuberculosis H37RV to establish tuberculosis model ,and the parameters including body weight, macroscopical visceral pathogenic degree, visceral weight index ( spleen , lung and liver ), the colony-forming unite(CFU) quantitative viscus culture for Mycobacterium tuberculosis in guinea-pigs were observed. Results ① The body weights in experimental groups with various doses of YLN (1.0 g/kg·d -1, 0.5 g/kg·d -1, 0.25 g/kg·d -1) were heavier than those of control group (P<0.05). In guinea pig , the effect of YLN combined with INH were superior to that of INH (P<0.05), ②The macroscopical visceral pathogenic degree of all groups treated with YLN decreased obviously (P<0.05). ③ Except for the spleen and liver weight indexes in low-dose group, the indexes of all groups treated with YLN were obviously lower than those of control group (P<0.05). ④ The CFUs of all groups treated with YLN except for low-dose group were obviously lower than those of control group (P<0.05). Conclusion YLN capsule has an anti-tuberculosis and anti-infection effect in guinea pig infected with M. tuberculosis H37RV,especially in the high-dose and the middle-dose YLN, which has a synergetic effect with INH.

关 键 词：杨氏欣痨宁 结核病 分支杆菌 豚鼠 

分 类 号：R-332[医药卫生] R978.3