Wistar大鼠实验性变态反应性脑脊髓炎模型的制备  

The making of experimental autoimmune encephalomyelitis model in Wistar rat

作　　者：刘颖[1] 刘华[1] 辛晋敏[1] 马太花[1] 梁丽云[1] 马存根[1] 

机构地区：[1]大同大学脑科学研究所,037008

出　　处：《山西医药杂志》2005年第3期189-190,共2页Shanxi Medical Journal

基　　金：山西省回国留学人员科研基金资助项目(2 0 0 3 6 9)

摘　　要：目的　探讨Wistar大鼠诱导实验性变态反应脑脊髓炎 (EAE)动物模型 ,检测EAE中起关键趋化作用的巨噬细胞趋化因子 - 1(MCP- 1)的表达。方法　采用免疫诱导方法制备EAE模型并苏木素 伊红 (HE)染色 ,同时用原位杂交法检测EAE大鼠MCP -1的表达。结果　免疫后 12～ 17d ,发病鼠出现EAE临床症状 ,HE染色 ,光镜下可见血管周围炎性浸润 ,MCP- 1的表达明显增加。结论　Wistar大鼠成功的诱导实验性变态反应脑脊髓炎模型是可信、可用的。Objective Establishing experimental autoimmune encephalomyelitis (EAE) model in insensitive Wistar rats from Shanxi Medical University to observe expression of MCP-1 mRNA.Methods With complete antigen and PT to immunize Wistar rats,and inoculate for 12～17 days;their brains and spinal cords were removed for HE staining and in situ hybridization (ISH).Results The animal got the clinical symptom of EAE;the characteristic light microscopical findings:perivascular inflammatory infiltration,and increased MCP-1 expression.Conclusion The EAE model of Wistar rats made up with the method is stable and reliable.

关 键 词：EAE WISTAR大鼠 MCP-1 表达 实验性变态反应性脑脊髓炎 诱导 免疫后 法制 目的 

分 类 号：R-332[医药卫生]