噬菌体多肽phage20抗胃癌肝转移作用的实验研究  

The inhibitive effect of selected peptide phage20 on liver metastasis of gastric cancer

作　　者：呼圣娟[1] 郭新宁[2] 杨力[2] 赵进[1] 张东涛[2] 翟慧红[2] 吴开春[3] 樊代明[3] 

机构地区：[1]宁夏银川市第一人民医院,宁夏银川750001 [2]宁夏医学院附属医院,宁夏银川750004 [3]第四军医大学西京医院全军消化病研究所,陕西西安710032

出　　处：《宁夏医学杂志》2005年第3期147-149,共3页Ningxia Medical Journal

基　　金：国家自然科学基金资助项目 (项目编号 :30 1 60 0 33)

摘　　要：目的　鉴定选择性噬菌体多肽phage2 0是否具有抗胃癌肝高转移细胞XGC9811-L肝转移的作用。方法　采用胃浆膜下裸鼠原位接种转移模型 ,观察phage2 0对胃癌细胞XGC9811-L肝转移能力的影响。裸鼠随机分为实验组 (phage2 0孵育组 12只 ) ,对照组 (XGC9811-L组 12只、M13wt孵育组 12只 ) ,原位接种后 10天、5周解剖裸鼠 ,观察肝转移率、转移瘤的数目及原发瘤的体积。结果　在原位接种后 10天 ,各组未发现肝转移灶 ,接种后5周phage2 0孵育组、XGC9811-L未孵育组、M13孵育组肝转移率分别为 :2 0 % ,10 0 % ,80 % ;肝转移灶的数目为 0 ,9.5± 2 .8,7.1± 4 .71;原发瘤的体积为 0 .6 5± 0 .4 32 ,0 .5 2 8± 0 .2 96 ,0 .5 82± 0 .348。与对照组相比phage2 0孵育组的肝转移率和转移灶的数目明显减少 ,P <0 .0 5 ,但在原发瘤的体积方面 3者间无显著差别 P >0 .0 5。结论　phage2 0可抑制胃癌肝高转移潜能细胞XGC9811-L肝转移能力 ,但对其生长无影响。Objective To identify whether selected pe pt ide phage20 had the ability to inhibit the potential of liver metastasis of gast ric cancer cells, XGC9811-L.Methods Metastasis model simu lating the metastatic manner of human gastric cancer was established by orthotop ic implantation of XGC9811-L suspension into the subserosa of stomach of nude mi ce. Mice were randomly divided into the experimental (phage20 preincubation grou p) and control groups (GC9811-L group and M13 wt preincubation group). At the in dicated time after inoculation,mice were sacrificed to observe the incidence of liver metastasis, the number of the metastasis foci, and the volume of primary tumor. Results There were no liver metastasis foci at 10 days after transplantation. While compared with the control groups, the incidenc e of liver metastasis was decreased significantly in experimental group at 5 wee ks after transplantation (20% vs 80% ,100% respectively, P<0.05). The numb er of liver metastasis foci were also decreased significantly in the experimenta l group(0 vs 9.5±2.8,7.1±4.71(P<0.01).But the volume of primary tumor h ad no difference between them (0.65±0.432 vs 0.528±0.296,0.582±0.348(P>0 .05).Conclusion Phage20 can inhibit the potential of live r metastasis ability of gastric cancer cell line, XGC9811-L. But it can not affe ct the growth of the target cells.

关 键 词：胃癌 肝转移 phage20 XGC811-L 

分 类 号：R735.2[医药卫生—肿瘤]