固相萃取-高效液相色谱法测定烟草样品中10种多酚  

Study on Solid Phase Extraction and High Performance Liquid Chromatography Method for the Determination of Ten Polyphenols in Tobacco

作　　者：张甜[1] 董学畅[1] 吴方评[1] 杨光宇[2] 乔永峰[1] 

机构地区：[1]云南民族大学化学与生物技术学院,昆明650031 [2]云南烟草科学研究院,昆明650106

出　　处：《分析化学》2005年第3期359-362,共4页Chinese Journal of Analytical Chemistry

基　　金：云南省烟草专卖局科研基金资助项目(No.2000B201)

摘　　要：研究了用固相萃取预分离,高效液相色谱法测定烟草样品中的10种植物多酚.烟草样品中的多酚提取液用Sep-Park-C18 固相萃取小柱预分离脱脂,以Waters Nova-Pak-C18 (3.9 mm×150 mm,5 μm)色谱柱为固定相,0.05 mol/L磷酸二氢钾缓冲溶液和甲醇梯度洗脱为流动相,烟草中主要的植物多酚均达到基线分离;用紫外二级管矩阵检测器检测,得出各组分在其最大吸收波长下的色谱图,根据该色谱图的峰面积定量,并用紫外光谱图对烟草中主要多酚进行辅助定性.标准回收率为94%～105%; RSD为1.3%～1.5%.用该方法测定了烟草样品中的10种植物多酚,结果令人满意.A high performance liquid chromatographic ( HPLC) method for the determination of ten polyphenols in tobacco was studied. The polyphenols was extracted from tobacco sample by refluxed with 80% methanol, And then cleaned up by solid phase extraction on a Waters Sep-Pak-C-18 cartridge. Polyphenols were separated on a Nova-Pak-C-18 chromatographic column (3. 9 mm x 150 mm, 5 mu m). The mobile phase was methanol and 0. 05 mol/L potassium dihydrogen phosphate buffer solution on gradient elution at a flow rate of 0. 5 mL/min. Each polyphenol was monitored by photodiode array detector at its maximum absorption wavelength and ten polyphenols in tobacco can be simultaneously determined by this method. The recovery of tobacco polyphenols was 94 % similar to 105 %, and relative standard deviation was 1. 3 % similar to 1. 5 %. This method was applied to the determination of polyphenols in tobacco with good results.

关 键 词：色谱图 固相萃取-高效液相色谱法 植物多酚 分离 辅助 梯度洗脱 RSD 烟草样品 脱脂 测定 

分 类 号：TS47[农业科学—烟草工业]