稻梨孢抗嘧菌酯突变体B9R2的部分生物学性状  

Several biological characteristics of azoxystrobin-resistant mutant B9-R2 of Magnaporthe grisea

作　　者：张舒亚[1] 李红霞[1] 王建新[1] 张传清[1] 周明国[1] 

机构地区：[1]南京农业大学农业部病虫监测与治理重点开放实验室,江苏南京210095

出　　处：《南京农业大学学报》2005年第1期39-43,共5页Journal of Nanjing Agricultural University

基　　金：农业部"948"项目 (2001 227)

摘　　要：通过紫外诱变获得引起稻瘟病的稻梨孢抗嘧菌酯突变体B9 R2, 此突变体对嘧菌酯表现为高抗药水平。B9 R2的40个单孢子后代的抗药性状稳定, 但是随着在无药平板上转代和低温培养, 抗药水平和生长速率均下降, 第 10代的抗药水平和生长速率分别下降 22 6%和 39 6%; 4℃培养 30d后, 抗药水平和生长速率分别下降 19 1%和 29 4%。与野生亲本菌株B9相比, 抗药突变体产孢量和孢子萌发率分别下降 65 8%和 12 9%。B9 R2的黑色素产生能力和致病力与亲本B9菌株相同。嘧菌酯不抑制抗药突变体B9 R2黑色素的产生, 但能强烈抑制孢子的产生, 在 100μg·mL-1嘧菌酯的产孢培养基上其产孢量下降了 93 2%。盆钵试验表明, 在活体条件下突变体B9 R2对嘧菌酯也具有抗药性。A mutant,B9-R2,of Magnaporthe grisea obtained by ultraviolet radiation showed highly resistance to azoxystrobin.The resistance level to azoxystrobin and mycelial growth rate of 40 single-conidium progeny of mutant B9-R2 reduced by 22.6% and 39.6% respectively after 10 generations of subculture in the absence of fungicide,and decreased by 19.1% and 29.4% respectively after incubation at 4 ℃ for 30 d.The amount of conidia and spore germination of B9-R2 reduced by 65.8% and 12.9% against wild parent isolate B9,respectively.There was no significant difference between B9 and B9-R2 in melanin production and pathogenicity.Azoxystrobin did not inhibit melanization of B9-R2 ,but inhibited producing condia effectively,and it was found that the amount of condia of B9-R2 decreased by 93.2% at AEA media amended with 100 μg·mL -1 azoxystrobin.Azoxystrobin also failed to control blast of rice seedlings after inoculation with resistant mutant B9-R2 effectively in vivo.

关 键 词：稻梨孢 嘧菌酯 突变体 抗药性 生物学特性 

分 类 号：S481.4[农业科学—农药学]