北京油鸡胚胎成纤维细胞系建立与生物学特性研究  

Establishment and Characteristics of a Beijing Fatty Chicken Embryo Fibroblast Cell Line

作　　者：周向梅[1] 马月辉[1] 关伟军[1] 文杰[1] 李晗[1] 

机构地区：[1]中国农业科学院畜牧研究所

出　　处：《畜牧兽医学报》2005年第3期209-215,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30100132);基础性工作重大专项(2001DEA10006)

摘　　要：采取组织块直接培养法,对北京油鸡胚胎组织进行原代和继代培养,成功地建立了成纤维细胞系.并对培养细胞进行了形态学、细胞生长动力学观察,以及核型和乳酸脱氢酶、苹果酸脱氢酶的同工酶分析.结果表明,该细胞系的群体倍增时间(PDT)为24 h;细胞染色体中二倍体占主体,为76%～88%;乳酸脱氢酶、苹果酸脱氢酶同工酶电泳图谱与本室其它细胞系有明显差别;细菌、真菌、病毒、支原体检测呈阴性.该细胞系的建立,使北京油鸡这一国家重要种质资源在细胞水平上保存下来,也为基因组文库和体细胞克隆等研究提供了理想的生物材料.A Beijing fatty chicken embryo fibroblast cell line (NBLCHE2/2) was successfully established using the primary explant technique. Observations on morphology, dynamic growth and analysis of karyotype, isoenzymes of lactate dehydrogenase, malate dehydrogenase were carried out. The results showed that population doubling time of cells was 24 h; diploid cells were dominant of 76%～88%. The banding patterns of the isoenzymes of the two kinds of emzymes had significant difference between the Beijing fatty chicken embryo fibroblast cell line and other cell lines in our laboratory; tests for microbial conta mination of bacteria, fungi and yeasts, virus and mycoplasma were negative. The newly established cell line make the Beijing Fatty Chicken, a national important genetic resource preserved in cell level, as well as will provide an ideal experimental material for the genetic studies on the Beijing local chicken.

关 键 词：成纤维细胞 细胞系 乳酸脱氢酶 胚胎 鸡胚 PDT 细胞染色体 北京油鸡 生物学特性研究 继代培养 

分 类 号：S831[农业科学—畜牧学] S436.8[农业科学—畜牧兽医]