污水中氧化铁鞘细菌的分离及其酶学特性研究  

Study on the Isolation and Enzyme Characteristic of Fe^(2+) Oxidizing Sheathed Bacteria in Sewage

作　　者：佘晨兴[1] 许旭萍[2] 李惠珍[2] 

机构地区：[1]福建师范大学环境科学系,福建福州350007 [2]福建师范大学生物工程学院,福建福州350007

出　　处：《福建师范大学学报（自然科学版）》2005年第1期81-84,共4页Journal of Fujian Normal University：Natural Science Edition

基　　金：福建省自然科学基金资助项目(97019)

摘　　要：采用试管静止富集培养及生态模拟富集培养,成功地从100份水样中分离到32株鞘细菌.利用Winogradsky液体试管法和摇瓶法进行氧化铁鞘细菌的快速筛选,从中筛选出1株氧化活性最高的菌株FC9901.对铁氧化酶特性进行了初步研究,该酶作用的最适pH和最适温度分别为7.5和30℃.金属离子Ca2+、Mg2+、Zn2+对氧化活性有激活和稳定作用;Cu2+、Hg2+、Al3+则抑制酶的活性;Fe2+、K+、Na+对酶活性影响不明显.32 strains of sheathed bacteria were successfully isolated from 100 water samples by two enrichment methods. Rapid screening of sheathed bacteria with Fe^(2+) oxidizing ability was done by Winogradsky liquid tube methods and flask methods. FC9901 was the best one which can produce oxidizing enzyme with the highest activity. Biochemical characteristic of the iron oxidizing enzyme were examined as follows, the optimum temperature and pH for oxidizing activity was 30℃ and 7.5. Ca^(2+) ?Mg^(2+) ?Zn^(2+) increased the oxidizing activity ,whereas Cu^(2+)?Hg^(2+) ?Al^(3+) caused inhibition .Fe^(2+)?K^(+) ?Na^(+) effected on the activity was not obvious.

关 键 词：氧化铁鞘细菌 分离 酶学特性 

分 类 号：Q935[生物学—微生物学]