Bax和Bcl-2在恒河猴正常妊娠和药物流产胎盘组织中的表达研究  

The Expression of Bax and Bcl-2 mRNA in the Placenta of Rhesus Monkey in Normal Pregnancy Group and Medical Abortion Group

作　　者：高红军[1] 代解杰[1] 唐东红[1] 王红星[2] 林海燕[2] 祝诚[2] 

机构地区：[1]中国医学科学院/中国协和医科大学医学生物学研究所,昆明650118 [2]中国科学院动物研究所计划生育生殖生物学国家重点实验室

出　　处：《中国计划生育学杂志》2005年第3期166-169,共4页Chinese Journal of Family Planning

基　　金：生殖生物学国家重点实验开放基金资助

摘　　要：　目的:Bax和Bcl-2是重要的凋亡调节因子,本研究主要探讨细胞凋亡与流产的关系。方 法:应用RT-PCR和原位杂交的方法检测恒河猴正常妊娠和药物流产胎盘、子宫中Bax和Bcl-2的 表达。结果:BaxmRNA在流产胎盘中的表达量明显高于正常妊娠胎盘,在胎盘绒毛滋养层细胞和基 底层蜕膜细胞均有明显表达。Bcl-2mRNA在流产胎盘中的表达量明显低于正常妊娠胎盘,表达模 式与Bax类似。结论:药物可能通过上调Bax和下调Bcl-2的表达,导致恒河猴胎盘组织和子宫基 底层凋亡细胞的数量增加,影响胎盘的正常结构和功能,从而增加了流产的危险性。Objective: To probe the relationship between cellular apoptosis and abortion. Methods: Bax and Bcl-2 mRNA were detected in placenta and uterus of both normal and treated groups, using RT-PCR and in situ hybridization. Results: The mRNA of Bax and Bcl-2 were identified in the trophoblast cells of placenta and deciduas cells of basal line. The expression of Bax mRNA in the placenta of the normal group was significantly lower than that of the treated group, whereas the expression of Bcl-2 mRNA in the placenta of normal group was significantly higher than that of treated group. Conclusions: The drugs might increase the quantity of apoptosis cells of placenta and basal layer of uteri by downregulating the expression of Bcl-2 mRNA and upregulating the expression of Bax mRNA, which influence the natural structure and function of placenta, and the possibility of abortion increased.

关 键 词：表达量 正常妊娠 恒河猴 药物流产 胎盘组织 BCL-2 子宫 RNA 结构和功能 表达模式 

分 类 号：R361[医药卫生—病理学]