条斑紫菜营养细胞的分离培养试验  

ON THE CULTIVATION OF THE ISOLATED VEGETATIVE CELLS OF PORPHYRA YEZOENSIS UEDA

作　　者：赵焕登[1] 张学成[1] 

机构地区：[1]山东海洋学院生物系

出　　处：《水产学报》1984年第3期197-202,共6页Journal of Fisheries of China

摘　　要：本文介绍了用条斑紫菜的单个营养细胞培养成叶状体的试验。试验表明,单个营养细胞,在适宜的培养液中有可能经过20天培养成小紫菜叶状体;其成活率为33％左右。此外,还介绍了冷冻度夏后的叶状体单个营养细胞的培养试验和不同含水量的叶状体营养细胞的培养试验。Individual vegetative cells of Porphyra yezoensis were obtained by mechanicalsmashing. The isolated cells were cultured in enriched soawater and developed intonew fronds in 20 days. The survial rate was about 33%.In attemping to make uee of the totipotency of the vegetative cells of this alga tomeet the ever increasing demand for seedlings in commercial culture. A series of experiments were carried out involving the freezing of the seaweed and keeping itat --20℃ for six months from June till December. Then the fronds were smashed toobtain the isolated vegetative cells, which could grow into normal frond seither inthe laboratory or in natural sea waters.Results of the experiments revealed that the survival rats of isolated cells isdirectly related to the water content of the fronds before freezing. When the watercontent 50% the survival rate was highest in this experiment.It seems possible by appling this principle to produce great amount of seedlingsto meet commercial need.

关 键 词：条斑紫菜 叶状体 试验 度夏 营养细胞 菜叶 养成 培养液 分离培养 

分 类 号：S968.431[农业科学—水产养殖] Q959[农业科学—水产科学]