几种细胞微囊化后致瘤性的研究  

Study of oncogenicity in several microencapsulated cells' xenograft

作　　者：陈冀衡[1] 杨茂元[1] 李继勇[1] 林献忠[1] 毕好生[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院麻醉学教研室

出　　处：《临床麻醉学杂志》2005年第3期185-187,共3页Journal of Clinical Anesthesiology

基　　金：国家自然科学基金资助(39900139)

摘　　要：目的　探讨微囊化技术对细胞异种移植致瘤性的影响。方法　应用免疫隔离技术原 理,制成了能将人体嗜铬细胞(HCC)、大鼠黑色素瘤细胞(B16 F1)及大鼠成纤维细胞(NIH3T3)与宿 主的免疫系统隔离的新型微囊。选雌性SD大鼠112只,随机分为14组,分别将APA空微囊、HCC、 微囊化HCC(ME HCC)、B16 F1细胞、ME B16 F1细胞、NIH3T3细胞、ME NIH3T3细胞移植到大 鼠眼前房和足胝部。每日观察大鼠的眼前房及足胝部,持续4周。定时取材行组织学观察。结果　 肉眼观察:B16 F1组和ME B16 F1组大鼠移植部位有新生物生长;B16 F1组的新生物重量和成瘤 率明显大于ME B16 F1组(P<0.01);其余各组未见新生物。光镜观察:B16 F1组移植后1周,移 植部位有薄层黑色素瘤细胞,第4周见较多黑色素瘤细胞;ME B16 F1组有3只大鼠移植部位有薄 层黑色素瘤细胞;余组未见瘤细胞。非微囊化细胞组大鼠移植部位见大量淋巴细胞浸润。空囊组和 微囊化细胞组大鼠移植部位仅见少量淋巴细胞。结论　移植细胞经微囊化技术处理后无致瘤性,说 明微囊化HCC异种移植是比较安全的,在生物镇痛领域内有广阔的应用前景。Objective To study oncogenicity in several microencapsulated cells'xenograft. Methods Human chromaffin cells(HCC),mice melanoma cells(B16-F1)and mice fibroblast cells(NIH3T3) were microencapsulated by immunoisolation technique,then 112 female Sprague-Dawley rats were randomly divided into 14 groups.These rats were injected into the anterior chamber of the eyes and the tendon of feet with APA hollow microcapsules or HCC or microencapsulated HCC(ME-HCC) or B16-F1 cell or ME-B16-F1 cell or NIH3T3 cell or ME-NIH3T3 cell. The anterior chamber of the eyes and tendon of feet were observed for 4 weeks and all rats were killed at last.Right eye balls and left feet were paraffin embed,then given morphology study.Results The anterior chamber of eyes and tendon of feet of these rats in B16-F1 and ME-B16-F1 transplantation groups were observed the neoplasm' growth one week later. The tumor weight and ratio of tumor formation of B16-F1 transplantation groups are significantly higher than that of ME-B16-F1 transplantation groups(P<0.01).This phenomenon didn't happen in other groups. Morphology: There were thin-layer melanoma cells one week later and multi-layers four weeks later in the anterior chamber of the eyes and tendon of feet in non-microencapsulated B16-F1 cells transplantation groups, There were thin-layer melanoma cells in transplant position in microencapsulated B16-F1 cells transplantation groups. but didn't grow in the other uncapsuled or microencapsulated cells groups.There were a number of lymphocyte and neutrophil in the anterior chamber of the eyes and tendon of feet in non-microencapsulated cell groups but there were only little lymphocyte and neutrophil in hollow microcapsules and microencapsulated cell groups. Conclusions Microencapsulated technique maybe eliminate neoplastic effect.Microencapsulated human chromaffin cells'xenograft is feasible and it can be wildly used in the field of analgesia.

关 键 词：微囊化 嗜铬细胞 体外培养 致瘤性 

分 类 号：R614[医药卫生—麻醉学]