改进的保存供体离体兔肺再灌注模型的建立  

Establishment of Isolated Rabbit Lung Reperfusion Model and Improvement of Donor Preservation

作　　者：叶卫华[1] 杨鸿生[1] 任琼辉 何建超[1] 刘长春[1] 孙嘉智[1] 

机构地区：[1]昆明医学院第一附属医院心胸外科,昆明650032 [2]云南省交通厅医院,昆明650000

出　　处：《昆明医学院学报》2005年第1期20-24,共5页Journal of Kunming Medical College

摘　　要：目的 :建立一个简单可靠的保存供体离体兔肺灌注模型 ,并将其应用于供肺保存研究 .方法 :将日本大耳白兔 12只作为供体 ,随机分为两组 ,肝素化后以高钾液处死 .建立改进的离体兔肺再灌注模型 ,其中A组 (n =6 ) ,供肺未经历温缺血 ,直接进行持续灌注 ;B组 ,供肺在室温下经历温缺血 1h ,然后给予再灌注 .监测再灌注前及再灌注期间的平均肺动脉压 (MPAP)、血气、湿干比等指标 .结果 :整个灌注期间 ,A组动、静脉血PvO2 、PvCO2 、MPAP及供肺湿干比均在正常范围 ;B组动脉血PaO2 低于A组 (P <0 . 0 1) ,湿干比高于A组 (P <0 . 0 5 ) .结论 :改进的新模型是供肺保存研究的简单。Objective: To develop an isolated rabbit lung reperfusion model and evaluate the function of donor lung after ischemic injury.Methods: 12 Japanese big ear rabbits used as donors were randomly divided into 2 groups.They were sacrificed with an intravenous injection of potassium chloride after heparinizated.A modified isolated rabbit model was established.Group A ( n =6), donor lungs were immediately perfused without ischemia; Group B: donor lungs were reperfused after room temperature ischemia.(1 hour ischemic internal).MPAP, blood gas and wet /dry weight ratio were evaluated.Results: In group A, PvO 2,PvCO 2,MPAP and W/D ratio were within the normal ranges and stably throughout the reperfustion.In group B, donor lungs showed significantly worse in PaO 2 and wet / dry ratio.Conclusion: This isolated model provides a more simple and reliable means for the research of lung preservation .

关 键 词：离体 再灌注模型 供体 缺血 保存 PAO2 正常范围 日本大耳白兔 CO2 应用 

分 类 号：R743[医药卫生—神经病学与精神病学] R657[医药卫生—临床医学]