小麦根质膜H^+-ATPase水解活性与吸钾关系研究  

Relation between H^+-ATPase hydrolytic activity and Potassium uptake in the plasma membrane of wheat roots

作　　者：化党领[1] 介晓磊[1] 郭天财 黄晓书 韩锦峰[1] 

机构地区：[1]河南农业大学农学院 [2]国家小麦工程技术研究中心 [3]河南省农业生物和工程技术重点开放实验室,河南郑州450002

出　　处：《干旱地区农业研究》2005年第2期109-114,共6页Agricultural Research in the Arid Areas

基　　金：国家"十五"科技攻关重大项目"小麦优质高效生产技术研究与示范"(2001BA507A02)研究内容。

摘　　要：以不同基因型小麦根为材料,采用蔗糖不连续密度梯度法制备质膜微囊,研究了钾对质膜H+-ATPase水解活性的影响。研究表明:生长环境中钾胁迫会导致小麦根质膜H+-ATP酶水解活性增加,且水解活性受钾刺激也增加;生长环境中钾充足会导致此酶水解活性比钾亏缺时低,且水解活性受钾刺激而下降。表明环境钾水平可影响根内H+-ATP酶活性及其某些性状。植株K吸收量与根质膜H+-ATP酶水解活性及受钾刺激特性关系不明显。决定钾吸收量的因素很多。The discontinuous sucrose density gradient method was adopted to study the effects of potassium on H^+-ATPase hydrolytic Activity with the roots of different genotypes of wheat. The study of many different materials showed that potassium stress existing in the environment of wheat growth could result in the increase of H^+-ATPase hydrolytic activity in wheat plasma membrane as well the increase of the hydrolytic activity of different genotypes of wheat. H^+-ATPase hydrolytic activity where there existed sufficient potassium in the environment of wheat growth was higher than that where there existed potassium shortage in the environment of wheat growth, and potassium shortage could lead to the decrease of the hydrolytic activity of different genotypes of wheat. These indicated that environmental potassium level could affect the hydrolytic activity and other traits of H^+-ATPase in wheat roots. Plant potassium uptake did not have an obvious relation with H^+-ATPase hydrolytic activity in root membrane and plant response to potassium stimulation. There were many factors to determine how much potassium could be taken up.

关 键 词：小麦 质膜H^+-ATPASE 生长环境 吸收量 植株 性状 性比 质膜微囊 胁迫 酶水解 

分 类 号：S512.1[农业科学—作物学] Q945[生物学—植物学]