部分背根切断对备用背根节神经元CNTF、PDGF表达的影响  

Expression of CNTF and PDGF in Spared Dorsal Root Ganglion after Partial Dorsal Root Rhizotomy

作　　者：张连双[1] 章为[1] 王廷华[1,2] 徐新运[1] 赵秀军[1] 李永红[1] 周雪[1] 

机构地区：[1]四川大学华西基础医学与法医学院组织胚胎学与神经生物学教研室,成都610041 [2]昆明医学院神经科学研究所

出　　处：《四川大学学报（医学版）》2005年第2期176-179,共4页Journal of Sichuan University(Medical Sciences)

基　　金：纽约中华医学基金会 (CMB0 0 -72 2 )资助

摘　　要：目的　研究部分去背根对备用背根节神经元睫状神经营养因子 (CNTF)和血小板源性生长因子(PDGF)表达的影响。方法　制作猫备用背根模型 ,分别在 3d、7d及 14 d时取备用背根节用 CNTF抗血清、PDGF抗血清行免疫组织化学 ABC法染色。分别计数各组阳性神经元总数以及大、中小阳性神经元的数量。结果　两因子在备用背根节的大、中小神经元均有表达。 CNTF阳性神经元总数及中小阳性神经元数均在 3d时明显减少 (P<0 .0 5 ) ,7d、14 d时与正常比较无差异 ,大阳性神经元在各个时间段与正常相比无差异。PDGF阳性神经元总数及中小阳性神经元数在 3d、7d时均较正常显著减少 (P<0 .0 5 ) ,14 d时与正常无差异 ,大阳性神经元数各个时间段均无差异。结论　部分背根切断对备用背根节内不同神经元表达 CNTF、PDGF有不同的影响。Objective To investigate the expression of ciliary neurotrophic factor (CNTF) and platelet derived growth factor(PDGF) in spared dorsal root ganglion(DRG) after partial dorsal root rhizotomy. Methods Twenty adult cats were divided into four groups. Five cats were kept intact in the control group. Fifteen cats were subjected to bilateral root rhizotomy, and on the 3rd day, 7th day and 14th day after operation, they were sacrificed as subjects in the three experiment groups respectively (n=5 per group) and their DRGs (L 6) were taken. Immunochemical ABC method was used to detect the distribution of CNTF and PDGF-immunoreactivity neurons in those DRGs. The quantitative analysis was conducted to get the numbers of CNTF, PDGF-positive total, large, and medium-small sized neurons in each group. Results CNTF, PDGF-immunoreactants were distributed in large and medium-small sized neurons. The numbers of total and medium-small sized CNTF positive neurons were noted to be decreased on the 3rd day after operation (P<0.05), and no difference 7th and 14th days was seen when compared with control (P>0.05), but the large positive neurons showed no difference. The total and medium-small sized PDGF positive neurons were found decreased apparently on the 3rd day and 7th day, but there was no difference on the 14th day as compared with normal level. Large positive neurons displayed no change at every time. Conclusion Partial dorsal root rhizotomy exerts different influence on the expression of CNTF and PDGF for different neurons in spared DRG.

关 键 词：睫状神经营养因子 血小板源性生长因子 备用背根节 免疫组织化学 猫 

分 类 号：R361[医药卫生—病理学]