20(R)-人参皂甙Rg3对大鼠肝癌细胞的作用  

Anticarcinogenic Effect of 20(R)-ginsenoside Rg3 on Induced Hepatocellular Carcinoma in Rats

作　　者：李肖[1] 官泳松[1] 周翔平[1] 孙龙[1] 刘源[1] 贺庆[1] 富力 毛咏秋[3] 

机构地区：[1]四川大学华西医院放射科,成都610041 [2]大连富生天然药物开发有限公司 [3]四川大学华西医院肿瘤中心

出　　处：《四川大学学报（医学版）》2005年第2期217-220,共4页Journal of Sichuan University(Medical Sciences)

基　　金：教育部博士点基金 (2 0 0 3 0 610 0 90 );纽约中华医学基金会(No.82 -4 12 );四川省中医药管理局科研基金 (2 0 0 4B0 3 )资助

摘　　要：目的　探讨人参皂甙 Rg3对大鼠诱发性肝癌的作用及其机制。方法　建立 SD大鼠诱发性肝癌模型 ,经肿瘤供血动脉灌注不同剂量的 2 0 (R) -人参皂甙 Rg3(0 .375、1.5、6 .0 mg/ kg) ,磁共振成像 (MRI)技术测量治疗前、后肿瘤的体积变化 ,流式细胞仪 (FCM)和免疫组织化学方法检测肿瘤细胞的凋亡、增殖及坏死情况。结果　高剂量组对肿瘤体积的影响与对照组比较 ,其差异具有显著性 (P<0 .0 5 ) ;低、中、高三个治疗组诱导大鼠肝癌细胞的平均凋亡率 (% )分别为 11.0 8± 3.78、13.5 7± 3.34和 2 7.35± 16 .0 4 ,高剂量组较其它组差异显著 ;用药后测得 S期细胞的平均比率 (% )分别为 2 3.98± 9.4 4、19.73± 6 .6 2和 14 .0 9± 3.4 8,治疗组与对照组相比均有显著性差异 ,且药物剂量与其对肿瘤细胞的抑制作用呈正相关。高剂量组中增殖细胞核抗原 (PCNA)与肿瘤坏死因子 (TNF)的表达与对照组相比差异有显著性 ;治疗组组间 PCNA与 TNF的表达差异不显著。结论　经肿瘤供血动脉灌注人参皂甙 Rg3能明显的抑制肿瘤细胞增殖、有效的诱导肿瘤细胞凋亡、促进肿瘤组织坏死 ,并有剂量依赖性。Objective To explore the anticarcinogenic mechanism of 20(R)-ginsenoside Rg3 in induced liver tumor in SD rat. Methods Thirty-five SD rats with induced hepatocellular carcinoma were divided into a control group and 3 dosage groups according to the dosing levels of 20(R)-ginsenoside Rg3.The tumour volume was measured by MR imaging. The apoptotic rat and S-phase fraction and diploid of tumor cell were mensurted with flow cytometry. Protein expression of PCNA and TNF were evaluated with immunohistochemistry. Results There was significant difference in tumour volume between the high dosage group and the control group. The average apoptotic rates were 11.08±3.78,13.57±3.34,27.35±16.04 and the S-phase fractions were 23.98±9.44,19.73±6.62,14.09±3.48 in the low-, medium-,and high-dosage groups respectively. The apoptotic rate was significantly higher in the high-dosage group than in the medium-dosage group and low-dosage group. Before-after comparison showed that the anti-proliferative effects of 20(R)-ginsenoside Rg3 were significant in three treatment groups. The higher positive rats of protein expression with PCNA and TNF were significant difference in the high-dosage group compared to those in the low-dosage group. No significant difference between the medium-dosage group and the low-dosage group. Conclusion 20(R)-ginsenoside Rg3 can noticeably inhibit the proliferation of tumor cells, and efficaciously induce the apoptosis and facilitate necrosis of the tumor cells,and there appears to be a dose dependent effect.

关 键 词：人参皂甙RG3 肝癌 细胞凋亡 

分 类 号：R735.7[医药卫生—肿瘤]