维甲酸诱导骨质疏松模型大鼠的效果及机理  

The Effect of Retinoic Acid on Induction of Osteoporotic Model Rats and the Possible Mechanism

作　　者：许鹏[1] 郭雄[1] 张银刚[1] 李幼芬[1] 曹峻岭[1] 熊咏民[1] 

机构地区：[1]西安交通大学环境与疾病相关基因教育部重点实验室地方病研究所,西安710061

出　　处：《四川大学学报（医学版）》2005年第2期229-232,共4页Journal of Sichuan University(Medical Sciences)

摘　　要：目的　观察维甲酸诱导雌性大鼠骨质疏松模型的效果 ,并分析其机理。方法　 SD雌性大鼠用维甲酸80 mg/ (kg· d)连续灌胃 15 d,于停药后 0、30和 6 0 d,测量血清 Ca、P、BGP、E2 、IGF- 1、AKP和 TRAP含量、骨组织中胶原和蛋白多糖成分的含量和 BMD,并观察股骨骨形态计量学和骺板软骨组织学变化。结果　大鼠维甲酸诱导 15 d后 ,血清 E2 和 BGP水平下降 ,AKP、TRAP活性增高 ,BMD下降 ;股骨松质骨和皮质骨骨量减少 ,骺板软骨细胞数量减少。停药后 30 d维甲酸组松质骨和皮质骨仍表现骨量减少 ,血清 AKP虽较前降低仍显著高于正常组 ;骺板软骨细胞数量增加 ,血清 BGP升高 ,但仍明显低于正常组 ;血 Ca显著升高 ;停药后 6 0 d维甲酸组股骨松质骨骨量依然显著减少 ,但皮质骨骨量、血清 Ca、BGP、AKP、TRAP含量和 BMD等两组大鼠无明显差异。结论　维甲酸诱导骨质疏松大鼠模型的近期效果好 ,远期效果较差 ;骨量丢失松质骨比皮质骨明显而持久。Objective To observe the effect of retinoic acid on induction of osteoporotic model in rats and analyze the mechanism therein involved. Methods SD rats were treated with retinoic acid 80 mg/(kg·d) by gastrogavage for 15 days to induce osteoporosis and were killed in batches at 0, 30 and 60 days after drug withdrawal. The levels of Ca, P, BGP, E 2, IGF-1, AKP and TRAP in serum were assayed, the collagen and proteoglycan contents of bone and bone mineral density (BMD) were determined, and the morphological changes in cancellous and cortical bone and growth plate cartilage (GPC) of femurs from the experimental rats were observed. Results After 15 days of induction by retinoic acid, the serum E 2 and BGP contents of rats were obviously decreased, the activities of AKP and TRAP were significantly increased, and the levels of BMD were lowered. The masses of spongy bone and cortical bone of femurs from the rats were decreased, and the number of chondrocytes in GPC was reduced. At 30 days, after drug withdrawal, the masses of spongy bone and cortical bone of femurs from the osteoporotic model rats still showed reduction; the activities of AKP in serum were lower than those at 15 days after drug redrawal, but were still higher than those of normal group rats; the chondrocytes in GPC were increased, the serum BGP and Ca contents were increased; At 60 days, after drug withdrawal, only the masses of femoral spongy bone of the osteoporotic model rats continuously showed obvious reduction, the other indices, including BGP, E 2, AKP, TRAP and the masses of cortical bone, showed no significant difference between the two groups. Conclusion The short-term effect of retinoic acid on induction of rat's osteoporotic model was noticeable, but the long-term effect was not so good, and the bone loss of spongy bone existed longer and was more obvious than that of cortical bone.

关 键 词：骨质疏松 大鼠 维甲酸 骨组织 

分 类 号：R-332[医药卫生]