一种细菌金属β-内酰胺酶筛查方法的建立  

The Method of Screening Metallo β-lactamases in Bacteria

作　　者：付有荣[1,2] 周俊英[1,2] 常江[1,2] 郭清莲[1,2] 

机构地区：[1]武汉大学中南医院检验科,武汉430071 [2]武汉大学医学院医学检验系,武汉430071

出　　处：《医学新知》2005年第1期43-44,共2页New Medicine

摘　　要：目的　建立一种简便、易行的实验方法 ,检测细菌产生的金属酶。方法　根据协同试验原理 ,采用亚胺培南 (IP)与巯基乙酸 (MA)协同作用的方法检测金属酶 ,并与PCR方法检测金属酶基因进行比较。结果　 4 7株嗜麦芽窄食单胞菌全部产生了金属酶 ;14株黄杆菌中有 9株产生了酶 ;2 0株铜绿假单胞菌均没有产生金属酶。该检测结果与PCR法检测金属酶基因结果一致。结论　使用伊米配能与巯基乙酸协同作用的方法可以检测金属酶 ,该方法有较好的敏感性和特异性 ,并且操作简便 ,不需要特殊的仪器设备 ,适合用于临床微生物实验室筛查产金属酶的菌株。Objective The purpose of this study is to set up a simpler method for detecting metallo β-lactamases from the clinical isolates. Methods Based on the synergy of imipenem(IP) and mercaptoacetic acid(MA), a filter disk was placed near one of the two KB disks of imipenem(IP) within a center-to-center distance of 15 to 20 mm. 10μl mercaptoacetic acid(MA), which is diluted by 1:10, was added to the filter disk. For metalloβ-lactamases-producing strains, there was evident change in the shape of the growth-inhibitory zone.Results All 47 strains of Xanthomonas maltophilia were metallo-β-lactamases. 9 out of 14 strains of Flavobacterium were metallo-β-lactamases. None out of 20 strains of Pseudomonas aeruginosa was metallo-β-lactamases.Conclusion The synergy method can be used for screening metallo -β-lactamase-producting Gram-negative bacteria in clinical laboratory.

关 键 词：金属酶 协同试验 伊米配能 巯基乙酸 

分 类 号：R446.5[医药卫生—诊断学]