ERIC技术在紫木耳亲缘关系鉴定上的应用研究  

APPLICATION OF ERIC METHOD TO THE AFFINITYAMONG AURICULARIA STRAINS

作　　者：温亚丽[1,2] 曹晖[3] 潘迎捷[3] 

机构地区：[1]南京农业大学生命科学学院微生物系 [2]上海市农业科学院食用菌研究所 农业部食用菌遗传育种重点开放实验室 上海市农业遗传育种重点开放实验室上海201106 [3]上海市农业科学院食用菌研究所农业部食用菌遗传育种重点开放实验室上海市农业遗传育种重点开放实验室

出　　处：《菌物学报》2005年第1期53-60,共8页Mycosystema

基　　金：上海市农业科学院发展基金项目

摘　　要：为了研究紫木耳与黑木耳和毛木耳之间的亲缘关系,利用ERIC分子标记、Southern杂交技术和形态学分类法,对紫木耳、黑木耳和毛木耳三者进行了详细的研究。在相似性系数为75%的水平上,由ERIC所得的聚类图将7个菌株分为2组,即黑木耳自成一组,毛木耳和紫木耳为一组,两者相似性系数为77%。Southern杂交实验证明毛木耳和紫木耳之间存在较高的同源性。子实体的形态学特征表明紫木耳与毛木耳的亲缘关系较之黑木耳更为密切,同时进一步说明ERIC分子标记是准确可信的。本研究探讨了紫木耳的分类地位,认为在基因组进化上,紫木耳与毛木耳的关系较之黑木耳更为密切。Enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) 、 Southern hybridization and morphological taxonomy methods were used to determine the affinities between of Auricularia sp., A. auricula and A. polytricha. Base on the 75% similarity coefficient, the dendrogram from ERIC-PCR exhibited two distinct parts, one representing A. auricula and the other containing A. polytricha and Auricularia sp..The similarity coefficient between A. polytricha and Auricularia sp. is 77%. The high homology relationships between A. polytricha and Auricularia sp. were reconfirmed by Southern hybridization. Morphological characteristics of fruiting bodies distinctly revealed that Auricularia spp. has a closer relationship with A. polytricha than to A. auricula, further indicating that ERIC method is authentic and convincible. The preliminary results suggested during the genome evolution, Auricularia spp. has a much closer relationship to A. polytricha than to A. auricula.

关 键 词：毛木耳 黑木耳 亲缘关系 相似性系数 分子标记 子实体 同源性 SOUTHERN杂交 形态学 鉴定 

分 类 号：S646.6[农业科学—蔬菜学]