雅致放射毛霉液体培养工艺及其多糖的免疫调节作用研究  

ACTINOMUCOR ELEGANS LIQUID CULTIVATION AND POLYSACCHARIDE IMMUNOMODULATION

作　　者：王谦[1] 靳发彬[1] 刘利群[1] 王丽英 王家槐 王永利[3] 张永健[3] 

机构地区：[1]河北大学生命科学学院,保定071002 [2]北京王致和食品集团有限公司,北京100039 [3]河北医科大学,石家庄050012

出　　处：《菌物学报》2005年第1期79-84,共6页Mycosystema

基　　金：河北省科技厅2001年科研项目(01245514D);河北省生物工程重点学科项目资助

摘　　要：对雅致放射毛霉液体培养研究表明,其适宜碳源为饴糖和可溶性淀粉,适宜氮源为黄豆粉和酵母膏,最适培养基含黄豆粉3%、饴糖0.5%、磷酸二氢钾0.2%、硫酸镁0.2%、酵母膏0.1%。适宜液体培养条件是温度28℃,接种量5%,发酵前期通风量1∶0.8,之后将风量调至1∶1.5~1∶2.0,全程不搅拌培养至18h^20h,生物量为每100mL发酵液中菌丝鲜重达30g以上。菌丝体氨基酸含量为46.1mg/100mL。免疫调节作用检验表明,雅致放射毛霉多糖具有一定的增强免疫力作用。Study on the liquid culture of Actinomucor elegans showed that the optimal carbon sources were maltose and soluble starch, and nitrogen sources were soybean flour and yeast extract .The optimal fermentation medium contained 3% soybean flour, 0.5% maltose, 0.2% KH2PO4 , 0.2% MgSO4·7H O,and 2 0.1% yeast extract. The biomass reached 30 g or more per 100mL fermentation solution at 28℃ for 18h^20h without stir with 5% inocula, and ventilation of 1:0.8 in earlier stage and 1:1.5-1:2.0 in later stage. The content of amino acid in mycelium was 46.1 mg·100mL–1. The immunomodulation tests showed that Actinomucor elegans polysaccharid had a certain effect on heightening immunity.

关 键 词：液体培养 鲜重 酵母膏 氨基酸含量 磷酸二氢钾 生物量 菌丝 免疫调节作用 适宜 多糖 

分 类 号：Q939.93[生物学—微生物学]